摘要
用来源于俄罗斯(E2),中国江西(JX2)、四川(SC)的3个拟松材线虫虫株,接种3年生黑松Pinus thunbergii,1个月后分离线虫体表携带的细菌。经API/ATB细菌自动鉴定系统鉴定:28个菌株中,鲁氏不动杆菌Acinetobacter 1woffii有10株,嗜水气单胞菌Aeromonas hydrophila和赫氏埃希菌Escherichia hermannii各有4株,其余10个菌株包括3株耳葡萄球菌Staphylococcus auricularis、2株泛菌Pantoeu agglomerans某些种、2株铜绿假单胞菌Pseudomonas aeruginosa、2株阴沟肠杆菌Enterobacter cloacae、1株荧光假单胞菌Pseudomonas fluorescens。对黑松切根苗的生物测定结果表明:强、弱、无致萎性菌株分别有10,15,3株。以强致萎活性菌SC5,SC7,E211和无致萎活性菌E25,JX23为代表,进行16S rDNA序列分析,结果表明:SC5为嗜水气单胞菌,SC7为荧光假单胞菌,E211为恶臭假单胞P.putida,E25为约翰逊不动杆菌Acinetobacter johnsonii,JX23为欧文氏菌Erwinia cypripedii。在此基础上,挑选SC7和E25为代表菌株,SC为代表虫株,对3年生黑松苗野外接种,结果为:强致萎病菌单独接种黑松不发病,无菌线虫单独接种也不发病,两者混合接种病情严重,说明拟松材线虫引起的松萎蔫病是该线虫与细菌复合侵染的结果。
Three Bursaphelenchus mucronatus （Bm） isolates collected from Russia（ named E2 ）, Jiangxi Province（ named JX2）, Sichuan Province （named SC） were inoculated into wild 3-year-old Pinus thunbergii seedlings. Bacteria strains on the body surface of Bm were isolated from those P. thunbergii branches one month later, then identified by API/ATB bacteria identification system. A total of 28 strains were determined, of which Acinetobacter lwoffii ,Aeromonas hydrophila, Escherichia hermannii were 10,4,4 respectively, and the rest 10 were other bacteria strains. In bioassay, 10,15,3 bacteria strains showed strong ,weak and non-pathogenicity ,respectively. The 16S rDNA sequence identification and analysis of representative strains including 3 strong-pathogenicity strains SC5, SC7, E211 and 2 non-pathogenicity strains E25, JX23 showed that： strains SC5 was Aeromonas hydrophila, SC7 was Pseudomonas fluorescens, E211 was P. putida, E25 was Acinetobacter johnsonii, JX23 was Erwinia cypripedii. On this basis, we selected SC7 and E25 as the representative strains for field inoculation on 3-year-old Pinus thunbergii seedlings. Results showed that both strong-pathogenicity bacteria strains and aseptic Bin（ABm） alone could not cause the wilting symptoms,but the pathogenic bacterial strain and ABm together resulted in pine wilt disease. It is therefore conluded that pine wilt disease resulted from the complex infection of Bm and their associated pathogenic bacteria.
出处
《中国森林病虫》
北大核心
2014年第4期5-10,共6页
Forest Pest and Disease
基金
国家林业局推广项目(编号:201004003)
关键词
拟松材线虫
生物测定
致病性
细菌
Bursaphelenchus mucronatus
bioassay
pathogenicity
bacteria
