摘要
目的:研究温胆汤对精神分裂症模型鼠血清肿瘤坏死因子(TNF-α)、白介素6(IL-6)及海马组织谷氨酸(Glu)活性表达的影响。方法:将72只清洁级SD大鼠随机分为6组:正常组(A)、模型组(B)、温胆汤高剂量组(C)、温胆汤中剂量组(D)、温胆汤低剂量组(E)、氯氮平组(F),每组12只。在造模前,C,D,E组分别ig温胆汤生药40,20,10 g·kg-1;F组ig氯氮平20 mg·kg-1;A,B组用等量生理盐水ig,1次/d,共21 d。在最后1次给药2 h后,B,C,D,E,F组一次性ip MK8010.6 mg·kg-1,建立精神分裂症模型,并进行刻板行为学观察;3 d后处死大鼠,取血清和海马组织检测。HE染色光镜下观察海马组织CA1区的病理改变,采用双抗体夹心-酶联免疫吸附(ABC-ELISA)法检测血清中TNF-α,IL-6的含量,免疫组化检测海马组织中Glu活性的表达。结果:与正常组比较,模型组大鼠出现刻板行为;血清TNF-α,IL-6含量明显升高(P<0.05或P<0.01);海马组织Glu蛋白表达水平显著降低(P<0.05或P<0.01)。与模型组比较,温胆汤各剂量组均不同程度改善了模型鼠的刻板行为和海马CA1区的病理改变,并可明显降低血清TNF-α,IL-6的含量(P<0.05或P<0.01),使海马组织Glu活性的表达也显著升高(P<0.05或P<0.01)。结论:温胆汤通过调节免疫功能、减少炎症因子产生和提高Glu活性的表达来减轻精神分裂症模型鼠海马组织的病理损害。
Objective： To study the effect of Wendan decoction on the contents of serum tumor necrosis factor-α （TNF-α） , interleukin-6 （IL-6） and the expression of glutamic acid （Glu） in hippocampus tissue in rats with schizophrenia. Method： Seventy-two SD rats were randomly divided six groups： normal group （A） , model group （B） , Wendan decoction high-dosage group （C） , Wendan decoction medium-dosage group （D） , Wendan decoction low-dosage group （E） , and Clozapine group （F） , twelve rats in each group. Before the model was established, the C, D, E groups were respectively given Wendan decoction （40, 20, 10 g·kg^-1）, the F group was given Clozapine （20 g·kg^-1 ） , the A and B groups were given normal saline every day, which last for 21 days. At the last given Wendan decoction or saline after two hours, the B, C, D, E, F groups were ip given MK801 0.6 g·kg^-1 to establish rat schizophrenia model, and stereotyped behavior of rats were observed. After three days, the rats were sacrificed, the serum and the hippocampus tissue were tested. To observe the CA1 regionof the hippocampus by microscope and HE staining. The contents of serum TNF-α, IL-6 were detected by double antibody clip ELASA method. The expression of Glu in hippocampus tissue was determined by immunohistochemical method. Result： Compared with A group, rats of B group appeared stereotyped behavior, the contents of serum TNF-α, IL-6 were significantly increased （P 〈 0.05 or P 〈 0. 01 ） and the expression of Glu in hippocampus tissue was decreased （P 〈 0.05 or P 〈 0.01 ） in rats of B group. Compared with B group, stereotyped behavior and pathological change of the CA1 region in hippocampus tissue were improved, Wendan decoction could significantly decrease the contents of serum TNF-α, IL-6 （P 〈 0. 05 or P 〈 0.01 ） and increase the expression of Glu in hippocampus tissue （P 〈 0.05 or P 〈 0. 01 ）. Conclusion： Wendan decoction could reduce the pathologic lesions of hippocampus tissue in rat with schizophrenia through regulating immune function, decreasing content of the inflammatory factor and increasing the expression of Glu.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第14期160-164,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
江西省卫生厅中医药一般计划(2012A156)
江西中医药大学重点学科青年教师培养计划(2012jzzdxk027)
关键词
温胆汤
精神分裂症
肿瘤坏死因子
白介素6
谷氨酸
Wendan decoction
schizophrenia
tumor necrosis factor-α
interleukin-6, glutamic acid
