摘要
目的观察姜黄素对人前列腺癌细胞株PC-3的体外放射增敏作用,并探讨其可能机制。方法不同浓度姜黄素以及不同剂量放射治疗作用于PC-3细胞,使用MTT法检测姜黄素毒性及放疗敏感性,克隆形成实验研究姜黄素对放疗敏感性的影响。姜黄素(浓度30μmol/L)联合放疗作用PC-3细胞24 h,流式细胞术检测各组细胞的凋亡率及细胞周期分布。结果姜黄素对人前列腺癌细胞株PC-3细胞有明显抑制作用,存在剂量和时间依赖性。姜黄素可降低放疗后PC-3细胞的克隆形成率,放疗增敏作用明显。姜黄素联合放疗组诱导细胞凋亡作用增强,且可将细胞周期阻滞在辐射敏感时相G2/M期。结论姜黄素对人前列腺癌细胞株PC-3的毒性呈剂量和时间依赖性,姜黄素可提高PC-3细胞放疗敏感性,可能与其引起肿瘤细胞周期阻滞和诱导细胞凋亡有关。
【Objective】To investigate the influence of sensibility of radiotherapy for PC-3 cells with curcumin.【Methods】The Human prostate cancer cell lines PC-3 was treated with different concentrations of curcumin and different intensity radiation dose, then the MTT assay was used to evaluate the cytotoxic effects of curcumin and radio-sensitivity on PC-3 cells. Clonogenic assay was employed to observe the effects on the radio-sensitivity of the PC-3.（30 μmol/L） curcumin with radiotherapy on PC-3 cells for 24 h. The flow cytometry was utilized to observe the apoptosis of PC-3 cells induced by curcumin and the cell-cycle of PC-3 cells in response to X-ray irradiation.【Results】The curcumin could significantly inhibit the proliferation of PC-3 cells, with obvious dose-and time-dependent effects. Subtoxic dose of curcumin at 30 μmol/L could significantly reduce the clonogenic activity. Irradiation combined with curcumin resulted in cell cycle arrest at G2/M phase in PC-3 cells. 【Conclusions】Curcumin can enhance the radio-sensitivity of the human prostate cancer cell lines PC-3 in vitro. The mechanism may involve the increased apoptosis and prolonged cell cycle arrest induced by the combined treatment.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2014年第18期27-30,共4页
China Journal of Modern Medicine
关键词
姜黄素
放射治疗
凋亡
敏感性
curcumin
radiotherapy
apoptosis
sensibility
