摘要
目的 研究连续6d睡眠剥夺对Wistar大鼠甲状腺功能及抗甲状腺抗体的影响.方法 健康3月龄Wistar大鼠30只,随机数字法分为睡眠剥夺组（SD组）、实验对照组（TC组）、空白对照组（CC组）,每组雌鼠、雄鼠各5只,利用“小平台水环境法”建立大鼠睡眠剥夺模型,以大平台及正常笼养组分别作为实验对照和空白对照.将SD组和TC组大鼠置于24 h拟光照环境中,对SD组大鼠进行连续6d睡眠剥夺;CC组模拟正常作息时间,饲养在每天12h拟光照、12h黑暗的环境中.6d后股动脉放血处死所有大鼠,留取甲状腺组织及血液,采用化学发光免疫分析法检测不同组大鼠血清总T3（TT3）、总T4（TT4）、游离T3（FT3）、游离T4（FT4）、促甲状腺激素（TSH）水平,采用放射免疫分析法检测血清甲状腺过氧化物酶（TPO）抗体、甲状腺球蛋白（Tg）抗体和Tg浓度.结果 SD组大鼠TT3水平较TC组和CC组明显升高（P均＜0.05）;TT4、FT4水平明显低于TC组和CC组（P均＜0.05）;FT3、Tg水平略有升高;TSH、TPO抗体、Tg抗体水平较TC组和CC组差异无统计学意义.结论 连续6d睡眠剥夺可影响大鼠甲状腺功能,但甲状腺功能尚可通过增加T4向T3转换而代偿.
Objective To study the effects of sleep deprivation for six-consecutive-day on thyroid function and anti-thyroid antibodies in Wistar rats.Methods Thirty rats aged 3 month healthy Wistar were randomly divided into three groups according to a random number table：sleep deprivation group（SD group）,tank cage group （TC group） and control group （CC group）,with five females and five males in each group.Sleep deprivation was induced in rats by housing them on small platforms over water.Controls were housed either in tanks with large platforms（TC group） or in normal cage（CC group）.Rats in SD group and TC group were placed in 24-hour proposed lighting environment,and rats in SD group were deprived of sleep for 6 consecutive days.Rats in CC group were placed in 12 hours light and 12 hours dark environment.Six days later,all rats were sacrificed,thyroid tissue and blood from femoral artery were saved.Total T3（TT3）,totalT4（T4）,free T3 （FT3）,free T4 （FT4） and thyroid stimulating hormone （TSH） in serum were detected by chemiluminescence immunoassay,and the level of anti-thyroid peroxidase（TPOAb）,anti-thyroglobulin （TgAb） and thyroglobulin （Tg） were evaluated by radioimmunoassay.Results TT3 in SD group were significandy higher than that of rats in TC and CC group （all P ＜0.05）.Compared with TC and CC group,the concentrations of TT4,FT4 were lower （all P ＜ 0.05）,and FT3 and Tg concentrations were a little higher.No significant changes were found in TSH,TPOAb and TgAb among the three groups.Conclusion Six consecutive days of sleep deprivation can influence thyroid function,but can be compensated by transferring more T4 to T3.
出处
《国际内分泌代谢杂志》
北大核心
2014年第4期225-228,共4页
International Journal of Endocrinology and Metabolism
基金
天津医科大学大学生学术研究资助计划
天津医科大学基础医学院大学生科研基金
关键词
睡眠剥夺
甲状腺功能
抗甲状腺抗体
大鼠
Sleep deprivation
Thyroid function
Antithyroid antibodies
Rats
