摘要
目的:研究芎芍胶囊对动脉粥样硬化(AS)兔主动脉核转录因子κB(NF-κB)及相关炎症因子的影响,探讨其抗炎性反应抗AS的新靶点。方法:60只新西兰白兔随机分为空白对照组、模型组、芎芍小剂量、中剂量及大剂量组、辛伐他汀组,采用单纯高脂喂养法复制兔AS模型。给药前及给药6、12、15周后分别采集血样检测血清中白介素-2(IL-2)、白介素-6(IL-6)及肿瘤坏死因子-α(TNF-α)水平;15周后处死兔取胸主动脉采用非放射性凝胶迁移实验(EMSA)法测定其NF-κB p65活性表达,采用实时定量PCR测定NF-κB mRNA表达量。结果:造模给药15周后各组兔血清炎症因子均较前不同程度升高,芎芍中、大剂量组及辛伐他汀组较模型组IL-2、TNF-α明显减少(P<0.01,P<0.05);芎芍大剂量组、辛伐他汀组较模型组NF-κB p65结合活性降低(P<0.05)。结论:芎芍胶囊能够降低AS兔主动脉NF-κB p65的蛋白结合活性,减少血清中相关炎症因子,从而通过抑制炎性反应达到抗AS的目的。
Objective： To study the effect of Xiongshao Capsule（XSC） on nuclear transcription factor κB（NF-κB） and inl ammation related factors of rabits with athrosclerosis（AS） and to investigate the new target for inhibiting the inflammatory response. Methods： Sixty New Zealand rabbits were randomly divided into control group, model group, low-dose XSC group, middle-dose XSC group, large-dose XSC group and simvastatin group. The rabbits of AS were established by simple high-fat feeding method. The levels of interleukin-2（IL-2）, interleukin-6（IL-6） and the tumor necrosis factors-α（TNF-α） in the serum were tested by blood tests after remodeling, before delivery of drugs, after 6, 12, 15 weeks of delivery, separately. The activity expression of NF-κB p65 in thoracic aortas was tested by electrophoretic mobility shift assay（EMSA）, and NF-κB mRNA was tested by Real-time quantitative PCR. Results： The serum inflammatory factors in medicated groups were increased to dif erent levels after 15 weeks. The IL-2 in middle-dose XSC group, high-dose XSC and simvastatin groups were lower than that in model group（P〈0.01, P〈0.05）, and TNF-α were signii cantly lower（P〈0.05）. The binding activity of NF-κB in simvastatin group was lower than that in model group（P〈0.05）. Conclusion： The XSC can lower the binding activity of NF-κB p65 in aortas of rabbits with AS and reduce the serum inl ammation related factors, which could prevent AS through anti-inflammatory response.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2014年第7期2334-2337,共4页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金资助项目(No.81173385)~~
