摘要
为了筛选高效降解羽毛角蛋白的芽孢菌株,对菌源样品通过以羽毛粉为唯一碳氮源进行富集培养,以酪蛋白固体平板初筛,测定菌株上清液中角蛋白酶活力并进行酪氨酸含量复筛,同时观察其对完整羽毛的降解速率,筛选到10株产角蛋白酶菌株,其中菌株JDBM-1 72h可将完整羽毛成分降解,角蛋白酶活力为117.39U/mL.综合菌株形态特征、生理生化试验结果和16SrRNA基因序列分析,确定JDBM-1菌株为Bacillus subtilis.
In order to screen high efficient feather-degrading stains,feathers were used as the sole carbon and nitrogen sources in the enrichment culture,casein medium was used in the primarily screen,keratinase activity and tyrosine content were determined in the supernatant of feathers degraded by screened stains,and the effects of complete feather degrading were observed.10 strains were screened out,one of the strains named JDBM-1 could degrade feather keratin completely in 72h,and its keratinase activity is 117.39U/mL.The bacteria strain of JDBM-1is identified as Bacillus subtilis by morphologic characteristic,physiology and biochemical properties,and 16SrRNA gene sequence analysis.
出处
《内蒙古大学学报（自然科学版）》
CAS
CSCD
北大核心
2014年第4期391-397,共7页
Journal of Inner Mongolia University：Natural Science Edition
基金
河北省科技支撑计划项目(项目编号:10225519)
关键词
废弃羽毛
角蛋白酶
分离筛选
枯草芽孢杆菌
wasted feather
caratinase
screening
identification
Bacilis subtilis
