摘要
目的分析实时荧光定量PCR检测HCV-RNA载量与化学发光免疫分析(CLIA)检测抗-HCV抗体的相关性。方法抽取CLIA法抗-HCV检测有反应性标本587例,采用实时荧光定量PCR进一步检测HCV-RNA。结果荧光定量PCR检测HCV-RNA,阴性225例,阳性362例,阳性率为61.67%,且阳性标本比例与CLIA法S/CO比值呈正相关。结论 HCV-RNA阳性率与CLIA法S/CO比值呈正相关,可根据S/CO比值预测HCV-RNA结果。
Objective To analyze the correlation of real-time fluorescent quantitation PCR（FQ-PCR）for detecting HCV-RNA loading and the chemiluminescence immunoassay（CLIA）for detecting anti-HCV antibody.Methods 587 samples of anti-HCV an-tibody positive detected by CLIA were furteher detected HCV-RNA by FQ-PCR.Results Among 587 samples of anti-HCV anti-body positive by the CLIA screening,225 samples were HCV-RNA negative and 362 samples were HCV-RNA positive detected by FQ-PCR,and the positive rate was 61 .67%,moreover,which was positively correlated with the S/CO ratio detected by CLIA.Con-clusion The positive rate of HCV-RNA is positively correlated with the S/CO ratio detected by CLIA.The result of HCV-RNA can be predicted according to the S/CO ratio.
出处
《国际检验医学杂志》
CAS
2014年第13期1749-1750,共2页
International Journal of Laboratory Medicine
关键词
肝炎抗体
丙型
化学发光测定法
聚合酶链反应
hepatitis C antibodies
chemiluminescent measurements
polymerase chain reaction
