期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

二甲双胍对LPS诱导THP-1细胞所产生的相关炎症因子的影响 被引量：5

下载PDF
导出
摘要 目的通过反转录聚合酶链反应(RT-PCR)技术,检测在二甲双胍(Met)及腺苷酸活化蛋白激酶(AMPK)的阻断剂复合物C(compound C)干预下,脂多糖(LPS)诱导的人单核细胞(THP-1)相关炎症因子白介素1β(IL-1β)、肿瘤坏死因子(TNF-α)基因水平的表达,探讨Met的抗炎作用及抗炎机制。方法体外培养人单核细胞,并分为对照组,LPS组(1μg/mL LPS),Met组(Met 5mmol/L+LPS 1μg/mL),复合物C组(Met 5mmol/L+LPS 1μg/mL+compound C 10μmol/L)分别孵育6h后,提取细胞进行检测。台盼蓝染色镜下计数观察细胞存活率。RT-PCR检测细胞炎症因子IL-1β、TNF-α的表达水平。结果 Met在干预6h后,可降低LPS所诱导的THP-1细胞的炎症因子IL-1β、TNF-α的基因表达。Met组炎症因子IL-1β、TNF-α的分泌显著低于LPS组(P<0.01)。复合物C组炎症因子IL-1β、TNF-α较Met组表达增加(P<0.01)。结论 Met干预6h可降低LPS诱导的THP-1细胞相关炎症因子IL-1β、TNF-α的基因表达,提示二甲双胍在基因水平上有一定的抗炎作用,且其抗炎途径与激活AMPK有关。
作者 李黛 刘云峰 梁栋 刘司漩 杨静
机构地区 山西医科大学第一临床医学院 山西医科大学第一医院
出处 《中西医结合心脑血管病杂志》 2014年第6期737-739,共3页 Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease
基金 国家自然科学基金资助项目(No.81270882)
关键词 二甲双胍 人单核细胞 苷酸活化蛋白激酶 炎症因子
分类号 R587.1 [医药卫生—内分泌] R255.4 [医药卫生—中医内科学]
  • 相关文献

参考文献15

  • 1Wellen KE,Hotamisligil GS. Inflammation,stress, and diabetes[J]. J Clin Invest, 2005,115(5):1111 - 1119.
  • 2Pickup JC,Mattock MB’Chusney GD,e( al. NIDDM as a disease ofthe innate immune system association of acute phase reactants andinterleykin - 6 with metabolic syndrome X[J], Diabeologia,1997,40(11):1286 - 1292.
  • 3Savage DB,Petersen KF,Shulman GL. Mechanisms of insulin re-sistance in humans and possible links inflammation[J]. Hyperten-sion,2005 >45(5):828 - 833.
  • 4Tilg H, Moschen AR. Insulin resistance,inflammation,and non -alcoholic fatty liver disease[J]. Trends Endocrinol Metab,2008,19(10):371 - 379.
  • 5Isoda K, Young JL. Zirlik A,et al. Metformin inhibits proinflam-matory responses and nuclear factor - kappaB in human vascularwall cells[J]. Arterioscler Thromb Vase Biol, 2006, 26 (3) : 611 -617.
  • 6Dagia NM,Goetz DJ. A proteasome inhibitor reduces concurrent,sequential,and long - term IL - 1 beta - and TNF - alpha - inducedECAM expression and adhesion[J]. Am J Physiol Cell Physiol,2003,285(4):C813-C822.
  • 7李慧,邹大进.2型糖尿病及肥胖与慢性炎症因子[J].第二军医大学学报,2003,24(11):1244-1246. 被引量：38
  • 8Diya Z, Lili C, Shenglai L^et al. LipopolysaccharideC LPS) of Por-phyromonas gingivalis induces IL - lbeta,TNF - alpha and IL - 6production by THP -1 cells in a way different from that of Esche-richia coli LPS[J]. Innate Immun.2008,14(2) :99 - 107.
  • 9徐丽,王芳,张宏.血管内皮黏附分子、超敏C反应蛋白及颈动脉内膜中层厚度的水平与2型糖尿病的相关性[J].广东医学,2010,31(5):595-596. 被引量：3
  • 10Hattori Y, Suzuki K, Hattori S,et al. Metformin inhibits cytokine -induced nuclear factor kappaB activation via AMP - activated pro-tein kinase activation in vascular endothelial celU[J], Hyperten-sion, 2006,47(6) : 1183 - 1188.

二级参考文献30

  • 1李振光,伍期专,唐朝枢,于占彩,王远臣,姚存姗,周慧杰,杨素国,于战涛.动脉粥样硬化患者颈动脉内膜中层厚度与血浆溶血磷脂酸含量的变化[J].中华医学杂志,2004,84(13):1071-1072. 被引量：20
  • 2张路,张凌鹏,吴宗贵.急性冠状动脉综合征病人VEGF与VCAM-1水平及其与斑块稳定性的研究[J].中西医结合心脑血管病杂志,2005,3(6):483-484. 被引量：1
  • 3王利民,蔡生业,姚成芳.黏附分子和动脉粥样硬化[J].心血管病学进展,2005,26(5):494-498. 被引量：9
  • 4KIM C S,PARK H S,KAWADA T,et al.circulating levels of MCP-1 and IL-8 are elevated in human subjects and associated with obesity-related parameters[J].Int J Obes(Lond),2006,30(9):1347-1355.
  • 5Barzilay JI, Abraham L, Heckbert SR, et al. The relation of markers of inflammation to the development of glucose disorders in the elderly[J]. Diabetes,2001,50(10):2384-2389.
  • 6Freeman DJ, Norrie J, Caslake MJ,et al. C-reactive protein is an independent predictor of risk for the development of diabetes in the West of Scotland Coronary Prevention Study[J].Diabetes,2002,51(5) :1596-1600.
  • 7Festa A, D'Agostino R Jr, Howard G,et al. Chronic subclinical inflammation as part of the insulin resistance sydrome. The Insulin Resistance Atherosclerosis Study (IRAS) [J]. Circulation,2000,102(1) :42-47.
  • 8Frohlich M,Imhof A,Berg G,et al. Association between C-reactive protein and features of the metabolic syndrome:a population-based study [J]. Diabetes Care, 2000, 23 ( 12 ) : 1835-1839.
  • 9Visser M, Bouter LM, Geraldine MM, et al. Elevated C-reactive protein levels in overweight and obese adults[J], JAMA,1999,282(22) :2131-2135.
  • 10Calandra T, Bernhagen J, Mitchell RA,et al. The macrophage is an important and previously unrecognized source of macrophage migration inhibitory factor[J], J Exp Med, 1994,179(6) :1895-1902.

共引文献39

同被引文献75

  • 1侯健,孔宪涛.环孢菌素A和FK506免疫抑制的分子机制[J].国外医学（药学分册）,1993,20(4):193-195. 被引量：3
  • 2徐以民,何俊琳,刘学庆,刘孝云,陈雪梅,王应雄.一种从子宫内膜高质量提取总RNA的方法[J].中国优生与遗传杂志,2006,14(5):39-40. 被引量：2
  • 3杨一新,杨云波,周鸣,周后德,向波,彭淑平,李小玲,沈守荣,李桂源.鼻咽癌细胞5-8F通过TLR4介导的信号通路对革兰氏阴性细菌脂多糖的反应性研究[J].生物化学与生物物理进展,2007,34(2):138-145. 被引量：2
  • 4Isoda K, Young J L, Zirlik A, et al. Metformin inhibits proinflam- matory responses and nuclear factor-kappaB in human vascular wall cells [J]. Arterioscler Thromb Vasc Biol, 2006, 26(3): 611 - 7.
  • 5Hyun B, Shin S, Lee A, et al. Metformin down-regulates TNF-cL secretion via suppression of scavenger receptors in macrophages [J].Immune Netw, 2013, 13(4): 123-32.
  • 6Sag D, Carling D, Stout R D, et al. Adenosine 5"-monophosphate- activated protein kinase promotes macrophage polarization to an an- ti-inflammatory functional phenotype [ J]. J Immunol, 2008, 181 (12) : 8633 -41.
  • 7Hattori Y, Suzuki K, Hattori S, et al. Metformin inhibits cyto- kine-induced nuclear factor kappaB activation via AMP-activated protein kinasc activation in vascular endothelial cells [ J ]. Hyper- tension, 2006, 47(6) : 1183 -8.
  • 8Kalender A, Selvaraj A, Kim S Y, et al. Metformin, independent of AMPK, inhibits mTORC1 in a rag GTPase-dependent manner [J]. Cell Metab, 2010, 11(5) : 390 -401.
  • 9Hulsmans M, Holvoet P. The vicious circle between oxidative stress and inflammation in atherosclerosis [ J ]. J Cell Mol Med, 2010, 14(1 -2) :70 -8.
  • 10Alhaider A A, Korashy H M, Sayed-Ahmed M M, et al. Metform- in attenuates streptozotocin-induced diabetic nephropathy in rats through modulation of oxidative stress genes expression [ J]. Chem Biol Interact, 2011, 192(3) : 233 -42.

引证文献5

二级引证文献59

中西医结合心脑血管病杂志
2014年 第6期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部