摘要
目的观察肠内营养支持对脑卒中患者体内血浆胰高血糖素样肽-1(GLP-1)水平及细胞免疫的影响。方法 85例脑卒中患者,随机分为肠内营养组(43例)和对照组(42例);肠内营养组给予肠内营养液(瑞代),对照组给予自制匀浆流食,两组患者均观察3周。采用酶联免疫法测定两组患者GLP-1;采用流式细胞仪检测患者CD3+(总T淋巴细胞)、CD4+(辅助性T细胞)、CD8+(毒性T细胞)和CD4+CD25+Foxp3+Treg细胞(调节性T细胞)。结果治疗后,肠内营养组血浆GLP-1与对照组比较显著升高(t=0.043,P<0.05);治疗后,肠内营养组CD3+、CD4+和CD4+CD25+Foxp3+Treg细胞明显增加(t分别=2.45、2.53、2.01,P均<0.05),GLP-1浓度与毒性T细胞亚群呈负相关(P<0.05)。结论肠内营养支持可提高脑卒中患者血浆中GLP-1浓度,且GLP-1的水平与毒性T细胞呈负相关。
Objective To investigate the effect of enteral nutrition（EN） on plasma glucagon- like peptide- 1 （GLP-1） and cellular immunity in stroke patients. Methods Eighty-five patients with stroke were randomly divid-ed into EN group（n=43） and control group（n=42）. Patients in EN group received enteral nutritional emulsion（Fre-subin Diabetes）and patients in control group were on self-made liquid diet,for 3 weeks. The level of GLP-1 was detected by ELISA. The level of CD3＋,CD4＋,CD8＋,and CD4＋CD25＋Foxp3＋Treg was determined by flow cytome-try. Results Compared with control group, patients in EN group had higher level of GLP-1 after treatment （t=0.043,P〈0.05）;the level of CD3＋,CD4＋,and CD4＋CD25＋Foxp3＋Treg was increased in EN group after treatment（t=2.45,2.53,2.01,Ps〈0.05）. The level of GLP-1 was negatively related to levels of T cell subsets（P〈0.05）. Conclu-sion EN can increase the concentration of plasma GLP-1 in stroke patients. The GLP-1 level is negatively related to toxic T cells.
出处
《浙江中西医结合杂志》
2014年第7期591-592,624,共3页
Zhejiang Journal of Integrated Traditional Chinese and Western Medicine
