摘要
目的 建立同时测定加杨树叶中的槲皮素、高良姜素、白杨素含量的分析方法。方法 采用高效液相色谱法,色谱柱Agilent TC C18柱（4.6 mm×250 mm,5μm）,流动相：甲醇-0.1%甲酸,梯度洗脱：0∽5 min,甲醇25%→55%,5∽25 min,甲醇55%→65%,25∽35 min,甲醇65%→75%;流速：1.0 mL.min 1;检测波长：264 nm;柱温：30℃。结果 加杨树叶中槲皮素、高良姜素、白杨素分离效果良好,线性范围分别为3.7∽120,2.5∽80和3.125∽100μg.mL 1;平均加样回收率分别为99.0%,100.1%,101.4%。结论 该方法准确可靠、结果稳定、重复性好,可为评价加杨树叶的质量提供依据。
OBJECTIVE To establish an analytical method for simultaneous determination of quercetin, galangin and chrysin in Populus canadensis Moench. leaves. METHODS The determination was performed on Agilent TC C18 column （4.6 mm×250 mm, 5 μm） with the mobile phase consists of methanol-0.1% formic acid at a flow rate of 1.0 mL-min-1 The following gradient elution was used： the content of methanol was 25% to 55% from 0 to 5 rain, 55% to 65% from 5 to 25 min and 65% to 75% from 25 to 35 min. The detection wavelength was set at 264 nm, and the column temperature was 30℃. RESULTS This method had a good separation performance for quercetin, galangin and chrysin in poplar leaves, and the linear range were 3.7-120, 2.5-80 and 3.125-100 μg·mL-1, respectively; the average recovery rates were 99%, 100.1% and 101.4%, respectively. CONCLUSION This method is accurate, reliable with good stability and repeatability which provides the evidence for quality evaluation for poplar leaves.
出处
《中国现代应用药学》
CAS
CSCD
2014年第7期857-860,共4页
Chinese Journal of Modern Applied Pharmacy
