摘要
目的研究黄独乙素(Diosbulbin B)对人胃癌SGC-7901细胞与其耐药细胞株的体外抑制作用。方法采用药物浓度递增法诱导人胃癌SGC-7901细胞,分别建立其顺铂、5-FU、阿霉素的耐药细胞株,应用MTT法检测黄独乙素对SGC-7901、SGC-7901/顺铂、SGC-7901/5-FU及SGC-7901/阿霉素的增殖抑制率,比较黄独乙素对以上四种细胞株的增殖抑制率是否有差异。结果黄独乙素对人胃癌SGC-7901细胞及其顺铂、5-FU、阿霉素耐药细胞株均表现出一定程度的剂量依赖性的增殖抑制作用(P<0.05),加入黄独乙素后人胃癌SGC-7901细胞的增殖抑制率与其顺铂、5-FU、阿霉素耐药细胞株的增殖抑制率近似(P>0.05)。结论黄独乙素对人胃癌SGC-7901细胞株表现出较低的交叉耐药性,能够在该细胞对顺铂、5-FU、阿霉素产生耐药后同样发挥增殖抑制作用。
Objective To research the inhibition of human gastric cancer SGC-7901 cells and drug-resistance cell strain in vitro by Diosbulbin B. Methods Induce human gastric cancer cell line SGC-7901 by drug concentration incremental method and set up its drug-resistance cell line of cis-platinum, 5-FU and adriamycin amycin respectively. Test the proliferation inhibition ratio of Diosbulbin B to SGC-7901, SGC-7901/cis-platinum SGC-7901/5-FU and SGC-7901/adriamycin amycin applying MTT method, at the meantime,compare the proliferation inhibition ratio between SGC-7901 and its drug-resistance cell strain. Results Diosbulbin B showed an effect of concentration dependent anti-proliferation within the scope of certainly concentration range to human gastric cancer cell line SGC-7901, SGC-7901/cis-platinum, SGC-7901/5-FU and SGC-7901/adriamycin （P〈0.05）, meanwhile, Diosbulbin B showed same effect within the scope of certainly concentration range to cis-platinum, 5-FU and adriamycin amycin drug-resistance cell strain of human gastric cancer cell line SGC-7901 （P〉0.05）. Conclusion Diosbulbin B show low cross resistance on the human gastric cancer cell line SGC-7901, and develop the same affect of proliferation inhibition when the cell line multidrug resistance with cis-platinum, 5-FU and adriamycin amycin.
出处
《医学研究与教育》
CAS
2014年第3期6-10,共5页
Medical Research and Education
