摘要
目的 研究地塞米松对人甲状腺滤泡上皮细胞凋亡的影响及其临床意义.方法 分离原代甲状腺滤泡上皮细胞,以0、10-6、10-5和10-4mol/L地塞米松刺激单层培养的甲状腺细胞,用MTT、流式细胞术及荧光定量PCR法检测刺激前后甲状腺细胞的增殖抑制率、凋亡率及凋亡相关基因mRNA的表达.结果 地塞米松在0～10-4mol/L浓度范围内可剂量依赖性地抑制甲状腺细胞增殖（P＜0.05）,增加甲状腺细胞凋亡率（P＜0.05）及凋亡相关基因mRNA的表达（P＜0.01）.结论 地塞米松可抑制甲状腺细胞增殖,促进甲状腺细胞凋亡的发生.
Objective To investigate the effect of dexamethasone on apoptosis of primary human thyroid follicular epithelial cells.Methods After separation and stimulation with different concentrations of dexamethasone,inhibition of proliferation,apoptosis and apoptosis-related mRNA expression of primary thyroid follicular epithelial cells were measured by MTT,flow cytometry and fluorescence quantitative PCR respectively.Results Within the concentration range of 10-6 ～ 10-4 mol/L,dexamethasone dose-dependently inhibited thyroid cell proliferation,increased cell apoptosis and expression of apoptosis-related mRNA of thyroid cells.Conclusions Dexamethasone can not only inhibit the proliferation of thyroid follicular epithelial cells,but also have effect on apoptosis of thyroid cells.
出处
《基础医学与临床》
CSCD
北大核心
2014年第7期950-954,共5页
Basic and Clinical Medicine
基金
南京市医学科技发展项目(ZKX12023)
关键词
地塞米松
甲状腺滤泡上皮细胞
细胞增殖
细胞凋亡
dexamethasone
thyroid follicular epithelial cells
cell proliferation
cell apoptosis
