摘要
目的分析基因型检测对诊断乙型肝炎病毒(HBV)感染程度的临床价值。方法选取某院2011年1月至2013年8月的HBV感染者433例,采用PCR-反向点杂交法检测感染者的HBV基因型,PCR法检测感染者DNA表达载量,ELISA方法检测HBV的E抗原。结果 HBV感染患者中B基因型患者比例(68.13%)明显高于BC混合基因型(5.77%)和C基因型(26.10%),差异有统计学意义(P<0.05);HBV患者DNA表达载量在不同基因型间差异无统计学意义(P>0.05);但HBV E抗原阴性率在不同基因型之间有所不同,B基因型23.82%,BC混合基因型14.78%,C基因型1.42%,差异有统计学意义(P<0.05);基因型与病情严重程度有关:轻中度乙型肝炎患者B基因型比例最高(87.20%),其次为C基因型(9.34%)和BC混合基因型(3.46%)、重度乙型肝炎分别为C基因型(77.08%)、BC混合基因型(14.58%)、B基因型(8.33%)。结论基因型与感染的严重程度及HBV E抗原阴性率有关,与DNA表达载量无关。
Objective To investigate the value of genotype detection for diagnosis of hepatitis B virus （HBV ） infection . Methods 433 HBV pattients from January 2011 to August 2013 were detected by PCR-reverse dot blot hybridization ;the DNA were assaied by PCR ;the HBeAg were tested by ELISA .Results Of the 432 HBV patients ,the rato of genotypes B （68 .13% ） was significantly higher than that of genotypes BC （5 .77% ） and of genotypes C（26 .10% ）（P〈0 .05）;there were no significiant differ-ence in the copy of the HBV DNA among the various genotype （P〉0 .05）;HBeAg negative rate of genotypes B （23 .82% ） ,geno-types BC（14 .78% ） ,genotypes C（1 .42% ）had statistically significant（P〈0 .05）;Genes associated with disease severity ：the ratio of genotype B for patients with mild-to-moderate hepatitis B was 87 .20% ,the rato of genotype C was 9 .34% and genotype BC was 3 .46% ,while the ratio of genotype C was 77 .08% ,genotype BC was 14 .58% ,genotype B was 8 .33% in severe hepatitis B .Con-clusion The genotype of HBV is related to disease severity and the negative rate of HBeAg ,it is not associated with HBV DNA of HBV .
出处
《重庆医学》
CAS
CSCD
北大核心
2014年第20期2557-2558,2562,共3页
Chongqing medicine
基金
湖南省科技计划资助项目(04FJ3024)
湖南省医药卫生科研计划项目(B2012-086)
关键词
乙型肝炎病毒
基因型
基因
反向点杂交
聚合酶链反应
hepatitis B virus
genotypes
gene
reverse dot hybridization
polymerase chain reaction
