摘要
目的探讨抑癌基因FRK(Fyn-related kinase)影响胶质瘤细胞侵袭和迁移的机制。方法将真核表达质粒pcDNA3.0-FRK和对照质粒pcDNA3.0转入人胶质瘤U25l细胞中,western blot技术检测FRK/N-cadherin/E-cadherin蛋白表达,细胞划痕试验检测细胞迁移能力,Transwell侵袭实验检测细胞侵袭能力。结果与对照组比较,转染pcDNA3.0-FRK质粒24 h后U25l细胞侵袭能力下降47%、迁移能力下降64%,差异均有统计学意义(P<0.01);转染pcDNA3.0-FRK可以明显增加N-cadherin/E-cadherin的表达。结论FRK可以通过增加N-cadherin/E-cadherin的表达,进而抑制胶质瘤细胞侵袭和迁移能力。
Objective To investigate the effect and mechanism of FRK（ Fyn-related kinase） on the human glioma cells migration and invasion. Methods Transfection of the pcDNA3. 0-FRK plasmid into the U251 glioma cell were carried out using Lipofectamine 2000. Wound healing assay and Transwell invasion assay were used to analyze the influence on the migration and invasion of glioma U251 ceils after transfection of the pcDNA3.0-FRK plasmid. Western blot was used to analyze the protein expressions of E-cadherin/ N-cadherin after FRK over-expression. Results Compared to the control plasmids, pcDNA3. O-FRK plasmid could increase the expression of FRK in glioma U251 cells In cell invasion assay, FRK inhibited cell invasion ability by 47%. In cell migration assay, FRK inhibited cell migration ability by 64%. Over-expression of FRK led to significant increase of E- cadherin/ N-cadherin protein expression levels in glioma U251 cells. Conclusion FRK may be inhibit glioma cells migration and invasion via increasing the expression of E-cadherin/ N-cadherin.
出处
《临床神经外科杂志》
CAS
2014年第3期169-171,共3页
Journal of Clinical Neurosurgery
基金
江苏省徐州市科技局(XZZD1155)
