摘要
目的从细胞超微结构水平了解大黄附子汤对百草枯中毒大鼠肺组织的干预作用。方法随机将雄性大鼠分为空白对照组、百草枯中毒组、阳性对照组、大黄附子汤组。按组制备模型,完成后处死大鼠并取材,取肺组织分别予HE染色观察病理变化、透射电镜观察细胞超微结构,以及检测肺组织湿/干质量比。结果 HE染色肺组织学评分显示百草枯中毒组较空白对照组升高明显,大黄附子汤组较百草枯中毒组下降,并低于阳性对照组(P<0.05),肺湿/干质量比也显示出相似结果。透射电镜结果观察百草枯中毒组肺损伤超微结构显示线粒体水肿,多数板层小体排空明显,形成空泡,微绒毛减少;阳性对照组显示上皮细胞轻度水肿,板层小体排空减轻,形成空泡数量减少;大黄附子汤组显示肺泡结构尚可,线粒水肿以及板层小体排空优于阳性对照组。结论大黄附子汤可改善百草枯中毒大鼠肺损伤的组织形态以及肺泡上皮细胞的超微结构。
Objective： To discuss the effect of Dahuangfuzi Decoction on lung uhrastructure of rats poisoned by paraquat. Methods： Male rats were randomly divided into 4 groups：blank control group,paraquat poisoning group,positive control group,and Dahuangfuzi Decoction group. Then the rats of every group were made into animal models. After that,samples were selected from the executed rats. Pathological change in lung tissue was observed by HE staining and ultrastructure and ratio of wet/dry weight by transmission electron microscope （TEM）. Results： Lung tissue grade in paraquat poisoning group was obviously higher than that in blank control group. Lung tissue grade in Dahuangfuzi Decoction group was lower than that in paraquat poisoning group and in positive control group （P 〈 0.05）. Lung wet/dry weight showed similar result. TEM of paraquat poisoning group showed swollen mitochondria, descreased microvillus, and obvious evacuation of lamellar body and cavitation bubble. Positive control group showed slightly swollen epithelial cells,decreased evacuation of lamellar body and decreased cavitation bubble. Dahuangfuzi Decoction group showed that structure of pulmonary alveoli was damaged slightly, and swollen mitochoudria and evacuation of lamellar body were less than those in positive control group. Conclusion： Dahuangfuzi Decoction can improve lung histomorphology and ultrastructure of alveolar epithelial cells of rats poisoned by paraquat.
出处
《中国中医急症》
2014年第7期1216-1217,1300,共3页
Journal of Emergency in Traditional Chinese Medicine
基金
国家自然科学基金资助项目(81173397/H2902)
辽宁省大连市卫生局课题资助项目(2010)
关键词
百草枯中毒
急性肺损伤
大黄附子汤
超微结构
Paraquat Poisoning
Acute lung injury
Dahuangfuzi Decoction
Ultrastructure
