摘要
目的:复制饮水型慢性氟中毒大鼠模型,观察模型大鼠大脑海马各区病理学改变及维生素E(VitE)拮抗氟中毒脑损伤的效果。方法:健康纯系SD大鼠36只,随机均分为对照组、染氟组及染氟-VitE拮抗组,雌雄各半,实验期10个月;观察大鼠氟斑牙发生情况,用氟离子选择电极法测定尿氟含量,利用光学显微镜观察各组大鼠脑组织海马病理学改变。结果:染氟组大鼠出现不同程度的氟斑牙,尿氟含量(2.35±0.51)mg/L明显高于对照组(1.50±0.14)mg/L,差异有统计学意义(P<0.05);染氟-VitE拮抗组氟斑牙程度及尿氟含量(1.95±0.12)mg/L均低于染氟组,差异有统计学意义(P<0.05);光镜下染氟组大鼠脑组织海马CA3、CA4区神经元胞浆及胞核未见明显改变,部分区域可见轻度排列紊乱;尼氏染色显示染氟组大鼠海马CA3、CA4区神经元内尼氏小体灰度值为(14.11±4.72)和(13.80±4.60)较对照组(70.06±7.77)和(73.55±5.94)及染氟-VitE拮抗组(37.56±8.68)和(44.54±8.52)明显减少,染色明显变浅,差异有统计学意义(P<0.01);染氟-VitE拮抗组较对照组灰度值也明显减少,差异有统计学意义(P<0.01)。结论:慢性氟中毒可使大鼠出现氟斑牙,尿氟含量升高;大脑海马神经元发生病理学改变,抗氧化剂VitE在一定程度上可降低慢性氟中毒引起的脑损伤。
Objective： To duplicate the animal model of chronic fluorosis by drinking water, and to observe the pathological changes in hippoeampus areas of rat brains and the antagonistic effect of vita- min E （VitE） on fluorosis. Methods： Thirty-six healthy SD rats were randomly divided into three groups： control group （group C）, fluoride group （group F）, fluoride exposing and vitamin E antago- nizing group （ group FE） , with half male and half female in each. The experiment period was 10 months. The dental fluorosis conditions were examined, and the fluoride contents in urine were deter- mined by fluoride ion selective electrode method. The pathological changes in cerebral hippoeampus areas were observed under light microscopy. Result： In group F, dental fluorosis with different degrees was found, and the fluoride contents in urine [ （2.35 ± 0.51 ） mg/L] were significantly higher than those in group C [ （1.50 ± 0.14） mg/L] （P 〈 0.05 ）. The degrees of dental fluorosis and urine fluor-ide contents in group FE [ （ 1.95 ±0.12） rag/L] were significantly lower than those in group F （P 〈 0.05 ）. No obvious difference was found in cytoplasm and nucleus of cells in brain hippocampus CA3, CA4 areas in group F, but slight arrangement disorder could be observed. Nisslg staining showed that grey degree values of Nissl ＇s body in the neurons of the hippocampus CA3, CA4 areas in group F [ （ 14.11 ± 4.72 ）, （ 13.80 ± 4. 60 ） ] were significantly lower than those in group C [ （ 70.06 ± 7.77）,（73.55 ~5.94） ] and group FE [ （37.56 ±8.68）,（44.54 ±8.52） ] （P 〈0.01）, and those in group FE were lower significantly than those in group C （P 〈 0. 01 ）. Conclusions： Chronic fluoro- sis can cause dental fluorosis, urinary fluoride increase, and pathological changes of cerebral hippocam- pus in fluoride rats. The antioxidants （VitE） can reduce brain injury induced by chronic fluorosis to some degree.
出处
《贵阳医学院学报》
CAS
2014年第3期290-293,301,共5页
Journal of Guiyang Medical College
基金
国家自然科学基金(81160335)
科技部国际合作重大项目(2013BAI05B03
2010DFB30530)
贵州省科技厅国际合作项目[黔科合外G字(2011)7014号]
关键词
氟化物中毒
海马
病理改变
牙
尿分析
fluoride poisoning
brain tissue
hippocampus
pathological change
tooth
urinalysis
vitamin E
rats, Sprague-Dawley
