摘要
目的:观察实验性慢性氟中毒大鼠脑组织中AKT激酶(AKT)表达及其学习记忆能力损害,探讨氟中毒大鼠脑组织中AKT蛋白表达水平与其学习记忆能力降低的关系。方法:SD大鼠72只,随机分为对照组、低剂量染氟组、高剂量染氟组,每组24只,雌雄各半,实验期6个月;观察氟中毒表现,Morris水迷宫检测实验大鼠学习记忆能力;分别用蛋白印迹(Western-blot)和逆转录-聚合酶链反应(RT-PCR)方法检测大鼠脑组织中AKT蛋白与AKT mRNA的表达。结果:经6个月饲养,低剂量及高剂量染氟组大鼠出现不同程度慢性氟中毒表现;Morris水迷宫实验中定向航行实验结果显示,高剂量染氟大鼠找台时间较对照组延长,空间探索实验显示氟中毒大鼠平台区停留时间较对照组减少(P<0.05);与正常对照组相比,高剂量及低剂量染氟组大鼠脑组织中AKT蛋白表达量增加(P<0.05),但脑组织中AKT mRNA表达水平与对照组相比差异无统计学意义(P>0.05)。结论:慢性氟中毒可导致大鼠学习记忆能力的降低,其机制可能与大鼠脑组织中AKT蛋白表达量增加有关。
Objective： To investigate the impairment of learning and memory of experimental rats with chronic fluorosis and expression of AKT kinase in rats＇brain tissues, to explore the correlation be- tween protein expression level of AKT kinase and reduced learning capcity. Methods： The 72 SD rats were grouped into three, 24 each, with equal distribution of genders, fed with different concentrations of fluoride （NaF） for six months to establish rat models with fluorosis. The spatial learning and memo- rizing ability were tested by Morris water maze test; the protein levels of AKT and expression AKT mR- NA were detected by Western-blotting and by PT-PCR respectively. Results： The reduced spatial learning and memory were detected in rats with chronic fluorosis in different levels; the group treated with high concentrations of fluoride require more time to find destination in Morris water maze test group, and stayed less time on platform zone in space exploration test as compared to control group （P 〈 0.05） ; increases in the expression of the protein levels of AKT were observed from both groups; comparison between expression level of AKT mRNA and control group are without statistical signifi- cance （ P 〉 0.05 ）. Conclusions： There is correlation between increased expression of AKT kinase and damage of learning and memory in rat brain with experimental chronic fluorosis.
出处
《贵阳医学院学报》
CAS
2014年第3期294-297,301,共5页
Journal of Guiyang Medical College
基金
国家自然科学基金(81260417)
科技部国际合作重大项目(2013BA105B03)
贵州省科技厅贵阳医学院联合基金[黔科合LG(2012)006号]
关键词
氟化物中毒
AKT激酶
脑
迷宫学习
记忆
fluoride poisoning
AKT kinase
brain
maze learnings
memory
rats, Sprague-Dawley
