摘要
目的小鼠进行重组蛋白与树突状细胞(DC)联合免疫,观察其对系统性白假丝酵母菌免疫保护效果。方法原核表达并诱导表达白假丝酵母菌Sap2蛋白;将白假丝酵母菌孢子、菌丝混悬液致敏小鼠DC后,分5组小鼠行3次免疫接种重组蛋白及致敏DC、致敏DC、DC、Sap2、PBS,再造系统性白假丝酵母菌感染模型观察小鼠生存期、肾病理切片、肾负菌量、检测CD4+、CD8+百分率观察保护性。结果成功构建pET32a-Sap2原核表达载体,目的蛋白被诱导表达并收集纯化。重组蛋白与致敏DC、DC、Sap2蛋白均能有效延长系统性白假丝酵母菌感染小鼠生存期、保护小鼠肾脏、增强小鼠的免疫力,其中多组分联合免疫效果更佳。结论多组分联合免疫能很好地抵抗系统性白假丝酵母菌感染,这为研究新型多组分联合疫苗奠定基础。
Objective To investigate the effect of combined immunization with recombinant Sap2 and dendritic cells （DCs） against systemic Canddida albicans infection. Methods We constructed a prokaryotic expression vector carrying sap2 of Candida albicans to obtain Sap2 protein. Murine DCs were sensitized by pulsing with Candida albicans spores and rope. Five groups of mice were immunized with recombinant Sap2 protein and sensitized DCs, sensitized DCs, naive DCs, Sap2, or PBS alone for 3 times, and the effect of the immunization against systemic Candida albicans infection were assessed by observing the survival of the mice, detecting the percentages of CD4^＋and CD8^＋cells, CFU in the kidney homogenate, and examining renal pathologies. Results Immunization with Sap2 and sensitized DCs and with DCs or Sap2 alone all prolonged the mouse survival and produced obvious effect in renal protection and immune enhancement, but such effects were more obvious with the combined immunization. Conclusion Combined immunization with Sap2 protein and DCs offers strong immunoprotection against systemic Candida albicans infection in mice, which provides experimental evidence for the development of new combined vaccines for immunoprotection.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2014年第7期1030-1034,共5页
Journal of Southern Medical University
基金
广东省自然科学基金(S2011010002599)
