摘要
目的探讨增殖性糖尿病视网膜病变(proliferative diabetic retinopathy,PDR)患者血浆、房水、玻璃体中血管内皮生长因子(vascular endothelial growth factor,VEGF)与组织纤溶酶原激活物(tissue type plasminogen activator,t-PA)及t-PA抑制剂(plasminogen activator inhibitor,PAI)的表达,并评价VEGF表达与t-PA、PAI的相关性。方法采用双抗体夹心酶联免疫吸附试验(ELISA)法定量检测来自宁夏医科大学总医院眼科的PDR组及对照组患者血浆、房水、玻璃体中VEGF、t-PA、PAI的含量。结果 PDR组血浆中VEGF(63.12±16.25)pg·mL-1、PAI(10.27±7.62)ng·mL-1的表达均高于对照组(14.39±10.48)pg·mL-1、(0.28±0.11)ng·mL-1,差异均有统计学意义(均为P<0.051;PDR组血浆中t-PA(19.91±9.90)ng·mL-1与对照组(12.00±2.61)ng·mL-1比较差异无统计学意义(P>0.01)。PDR组房水中VEGF(291.21±45.04)pg·mL-1、t-PA(5.38±2.40)ng·mL-1、PAI(0.53±0.31)ng·mL-1均高于对照组(30.60±11.70)pg·mL-1、(3.87±1.32)ng·mL-1、(0.16±0.09)ng·mL-1,差异均有统计学意义(均为P<0.05);PDR组玻璃体中VEGF(527.62±79.00)pg·mL-1、t-PA(10.41±6.33)ng·mL-1、PAI(7.72±2.32)ng·mL-1的表达浓度均高于对照组(37.58±8.31)pg·mL-1、(3.68±2.48)ng·mL-1、(0.27±0.13)ng·mL-1,差异均有统计学意义(均匀P<0.05)。PDR组自身血浆、房水、玻璃体三者比较,VEGF表达:玻璃体>房水>血浆;t-PA表达:血浆>玻璃体>房水;PAI表达:血浆、玻璃体均高于房水,但血浆和玻璃体比较差异无统计学意义(P>0.05)。PDR组血浆、房水、玻璃体中VEGF的表达与t-PA及PAI的表达均存在显著相关性(均为P<0.05)。结论 VEGF、t-PA、PAI可能参与了PDR视网膜新生血管的发生过程;VEGF与t-PA、PAI在PDR的发展中可能具有相互促进的作用。
Objective To evaluate the correlation between tissue-type plasminogen activator （ t-PA）, plasminogen activator inhibitor （PAI） and vascular endothelial growth factor （VEGF） expression in plasma aqueous humor and vitreous of proliferative diabetic retinopathy（PDR）. Methods The concentrations of VEGF, t-PA and PAI in plasma, aqueous humor and vitreous body in PDR and normal group were detected by ELISA. Results The levels of VEGF （ 63. 12 ±15.25 ） pg·mL^-1 and PAI（ 10.27 ± 7. 52）ng·mL^-1 in the plasma of PDR patients were higher than control group （ 14. 39 ± 10.48）pg·mL^-1 and PAI （0.28 ±0. 11 ）ng·mL^-1 ,and there were significant statistically differences （ all P 〈 0.01 ）. The levels of t-PA in plasma of PDR patients （ 19.91 ±9. 90）ng·mL^-1 and control group （ 12.00±2.61 ）ng·mL^-1 was no statistically different （P 〉0.05）. The levels of VEGF （291.21 ±45.04）pg·mL^-1 ,t-PA （5.38 ±2.40）ng·mL^-1 and PAI （0.53 ± 0.31 ）ng·mL^-1 in the aqueous humor of PDR patients were higher than control group （30.60 ± 11.70）pg·mL^-1, （3.87± 1.32）ng·mL^-1 and （0. 16 ± 0.09） ng·mL^-1 , and there were statistical differences （ all P 〈 0. 05 ）. The levels of VEGF（527.62 ±79.00）pg·mL^-1 ,t-PA （ 10.41 ±6.33）ng·mL^-1and PAI （7.72 ±2.32） ng·mL^-1 in the vitreous of PDR patients were higher than control group （37. 58± 8.31）pg·mL^-1,（3.68 ±2.48）ng ·mL^-1 and （0.27 ±0.13）ng·mL^-1 ,and there were statistical differences （ all P 〈 0.05 ）. Compared among plasma, aqueous humor and vitreous of PDR patients, the levels of VEGF in vitreous was more than that in aqueous humor,and aqueous humor was more than plasma;The levels of t-PA in plasma was more than that in vitreous, and vitreous was more than aqueous humor; The levels of PAI in plasma and vitreous were more than that in aqueous humor, and there was no statistical difference between plasma and vitreous （P 〉 0.05 ）. The expression of VEGF was highly correlated with t-PA and PAI in plasma, aqueous humor and vitreous of PDR （ all P 〈 0.05 ）. Conclusion VEGF, t-PA and PAI may be involved in the pathogenesis of angiogenesis in PDR, VEGF and t-PA PAI play interdependant roles in the development of PDR.
出处
《眼科新进展》
CAS
北大核心
2014年第7期647-651,共5页
Recent Advances in Ophthalmology
基金
宁夏自然科学基金资助(编号:NZ10129)~~
