摘要
目的研究β-微管蛋白Ⅲ(β-tubulinⅢ)在胃癌组织中的表达情况,探讨其表达与临床病理特征之间的关系。方法采用免疫组织化学的方法检测β-tubulinⅢ在50例胃癌组织及部分相应癌旁组织中的表达,结合患者的性别、年龄、肿瘤大小、部位、分化程度、Borrmann分型、浸润深度、淋巴结转移和TNM分期等临床病理参数进行综合分析。结果胃癌组织和癌旁组织中的β-tubulinⅢ蛋白阳性表达分别为31例(62.00%)、9例(40.91%),差异无统计学意义(χ2=2.752,P>0.05)。β-tubulinⅢ蛋白T1组阳性表达率为22.22%(2/9),T2组阳性表达率为37.50%(3/8),T3组阳性表达率为78.79%(26/33),三组阳性表达率比较差异有统计学意义(P<0.01,χ2=11.790),Spearman等级相关性分析显示,β-tubulinⅢ蛋白阳性表达与胃癌组织中浸润深度呈负相关(Rs=-0.481,P<0.05);β-tubulinⅢ在无淋巴结转移组中阳性表达率为38.89%(7/18),有淋巴结转移组中阳性表达率为75.00%(24/32),两者比较差异有统计学意义(P<0.05,χ2=6.376);β-tubulinⅢ蛋白阳性表达与患者的性别、年龄、肿瘤大小、部位、分化程度、Borrmann分型及TNM分期均无明显相关(P>0.05)。结论β-tubulinⅢ在胃癌中表达率稍高于癌旁组织,但差异不具有统计学意义。其表达水平与肿瘤浸润深度、淋巴结转移有关。
Objective This study was designed to delineate the expression and significance of β-tubulinⅢ in gastric cancer and to investigate the relationship between its expression and elinicopathologic fea- tures. Methods The expression of β-tubulinⅢ protein in 50 gastric cancer and partial paracareinoma tissues was detected by immunohischemistry and was comprehensively analyzed combined with patients＇ clinicopathologic parameters including gender, age, tumor diameter, tumor site, differentiation, Borrmann type, infiltration depth, lymph node metastasis and TNM stage. Results The expression of β-tubulinⅢ was positive in 31 gastric cancer （62. 00% ） and 9 （40. 91% ） paracarcinoma tissues with no statistical significance （X2 =2. 752, P 〉 0. 05 ）. β-tubulinⅢ expression was correlated with the depth of tumor infiltration. The positive rate of β-tubulinⅢ was 22.22% （2/9） in the T1 group, 37.50% （3/8） in the T2 group and 78.79% （26/ 33 ） in the T3 group with a statistical significance （ P 〈 0. 01, X2 = 11. 790 ）. The expression of β-tubulinⅢ was found to have positive correlations with the depth of tumor infiltration in gastric cancer by the Spearman rank test （R2 = - 0. 481, P 〈 0.05 ）. The positive rate ofβ-tubulinⅢ in the non-lymph node metastasis group was 38. 89% （7/18） and 75.00% （24/32） in the lymph node metastasis group with a statistical significance （ P 〈 0.05, X2 = 6. 376）. The expression of β-tubulinⅢ was not associated with patients＇ gender, age, tumor diameter, tumor site, differentiation, Bormlann type or TNM stage （ P 〉 0.05 ）. Conclusions The expression level of β-tubulinⅢ in gastric cancer tissues was higher compared with that in paracarcinoma tissues with no statistical significance. β-tubulinⅢ expression was correlated with depth of tumor infiltration and lymph node metastasis.
出处
《新医学》
2014年第7期444-447,共4页
Journal of New Medicine
基金
江苏大学医学临床科技发展基金(JLY20120104)
