摘要
目的:建立黄毛耳草的HPLC指纹图谱。方法:以Hypersil BDS C18(250 mm×4.6 mm,5μm)为色谱柱,甲醇-0.1%醋酸溶液为流动相进行梯度洗脱,检测波长345 nm,柱温35℃,以乌索酸为参照物得到含有9个峰的共有模式,以共有模式对不同产地黄毛耳草指纹图谱进行相似度分析。结果:从谱图相似度看出,不同产地黄毛耳草指纹图谱基本一致,但也存在地域性差异。结论:HPLC指纹图谱可用于黄毛耳草的鉴别和质量控制。
Objective ： To establish HPLC fingerprint of Hedyotis chrysotricha. Methods ： Hypersil BDS Ct8 （ 250 mm ~×4.6 mm,5μm） column was used with methanol and O. 1% acetic acid solution as mobile phase in gradient mode, the flow rate was 1.0 mL/ min. The detection wavelength was 345 nm and the temperature of the column was set at 30 ℃. Using ursolic acid as the reference standard,the mutual mode of Hedyotis chrysotricha containing nine common peaks was set up. The similarity of Hedyotis chrysotricha from different habitats was analyzed. Results：The fingerprints of Hedyotis chrysotricha from different habitats were similar,but also had habitat characteristics, Conclusion ： The HPLC fingerprint can be used for identification and quality control of Hedyotis chrysotricha.
出处
《中药材》
CAS
CSCD
北大核心
2014年第5期789-791,共3页
Journal of Chinese Medicinal Materials
基金
山东省高等学校科技计划项目(J11LB64)
山东省高校生物医学工程技术重点实验室资助课题
关键词
黄毛耳草
HPLC指纹图谱
Hedyotis chrysotricha （Palib.） Merr.
HPLC Fingerprint
