摘要
目的:优选裸花紫珠中总黄酮成分提取工艺方法。方法:采用正交试验以裸花紫珠中总黄酮的提取率为考察指标,考察提取时间、提取次数和水用量等3个因素对总黄酮提取率的影响,并考察醇沉除杂乙醇浓度。结果:裸花紫珠中总黄酮最佳醇提工艺为10倍量水煮提取3次,每次3h,提取液浓缩至1mL/g(干药材),以65%乙醇醇沉。结论:应用该工艺提取裸花紫珠中总黄酮提取转移率高,杂质较少,可为裸花紫珠中总黄酮提取工艺的确定提供实验依据。
Objective： To explore the optimum technology of extracting total flavonoids from Callicarpa nudiyTora Hook. et Arn. By orthogonal test. Methods： By orthogonal design L9 （3^4）, extraction times, extraction time and amount of water were investigated. In alcohol precipitation, the alcohol concentration was studied. Results： The optimum process was as follows： the crude drug was extracted three times （3 h each times） with water and the solid-to-liqiud ratio of 1 - 10. In alcohol precipitation, the alcohol concentration was 65 %. Conclusions： With high extraction rate of total flavonoids and low impurity of the extractum, this technology is simple and feasible. The study provides experimental basis for extraction of total flavonoids from Callicarpa nudiflora Hook. et Am.
出处
《海南医学院学报》
CAS
2014年第8期1044-1045,1049,共3页
Journal of Hainan Medical University
基金
浙江省公益技术研究社会发展项目(2012C23082)
海南省重点科技计划项目(ZDXM20120095)
海南医学院科研培育基金(HY2010-011)~~
关键词
裸花紫珠
总黄酮
正交试验
Callicarpa nudiflora Hook. et Arn.
Total flavonoids
Orthogonal test
