摘要
目的：对多叶越南槐中苦参碱与氧化苦参碱进行分离及含量测定。方法采用溶剂提取、萃取、硅胶色谱柱分离纯化，利用色谱技术进行化学结构鉴定；高效液相色谱（ HPLC）法色谱条件为：Kromasil 100-5 NH2（250 mm ×4．6 mm，5μm）色谱柱；流动相为乙腈-磷酸二氢钠（0．05 mol/L）-磷酸水溶液（3．0％）-乙醇（80∶5∶9．5∶5．5）；流速为1．0 ml/min；检测波长为210 nm；柱温为30℃。结果从多叶越南槐中分离得到两生物碱类化合物，经鉴定为苦参碱与氧化苦参碱；苦参碱在0．0164～0．1476μg范围与其峰面积呈良好的线性关系，氧化苦参碱在0．2872～2．5848μg范围与其峰面积呈良好的线性关系。苦参碱和氧化苦参碱的加样回收率分别为100．2％（RSD＝0．76％）、99．8％（RSD＝1．19％）。结论苦参碱与氧化苦参碱均为首次从多叶越南槐中分离得到；所建立的HPLC简便、准确、重现性好，结果准确可靠，可用于多叶越南槐中苦参碱与氧化苦参碱的含量测定。
Objective To study the isolation and assaying of matrine and oxymatrine in Sophora tonkinensis Gagnep.var.Polyphylla.Methods The chemical constituents were extracted by solvent and then isolated by silica gelchromatographycolumn .The chromatographic technique was used to identify its chemical structures .The chromatographic conditions of assaying by high performance liquid chromatography （ HPLC ） as follows：Kromasil 100-5 NH2 column （250 mm ×4.6 mm,5 μm） with acetonitrile-monometallic sodium orthophosphate （0.05 mol/L）-phosphoric acid solution（3.0%）-ethylalcohol （80 ∶5 ∶9.5 ∶5.5） as mobile phase,the flow rate was 1.0 ml/min,the UV detection wave length was 210 nm,and the column temperature was 30℃.Results The two alkaloids compounds attained from the separation of Sophora tonkinensis Gagnep.var.Polyphylla were identified as matrine and oxymatrine .The matrine formed linear relation with its peak area when it was in the range of 0.0164 -0.1476 μg.The oxymatrine formed linear relation with its peak area when it was in the range of 0.2872 -2.5848 μg.The average recovery rates of matrine and oxymatrine were 100 .2%（ RSD=0 .76%） and 99 .8%（ RSD =1 .19%） , respectively .Conclusion Matrine and oxymatrine are attained from the separation of Sophora tonkinensis Gagnep.var.Polyphylla for the first time.The established method（HPLC） in this study is simple,reliable,reproducible and accurate.It could be applied to the assaying of matrine and oxymatrine in Sophora tonkinensis Gagnep.var.polyphylla.
出处
《广西医学》
CAS
2014年第8期1109-1112,共4页
Guangxi Medical Journal
基金
广西自然科学基金资助项目(2010GXNSFA013135)
广西科学研究与技术开发计划项目(11107009-3-4)
关键词
多叶越南槐
苦参碱
氧化苦参碱
高效液相色谱
含量测定
Sophora tonkinensis Gagnep.var.Polyphylla
Matrine
Oxymatrine
High performance liquid chromatography
Assaying
