摘要
为了研究氨氮毒性作用对脊尾白虾(Exopalaemon carinicauda)抗氧化及脂质过氧化作用的影响,将脊尾白虾暴露于低浓度氨氮(4.79±0.12)mg·L-1、中浓度氨氮(9.05±0.18)mg·L-1、中高浓度氨氮(17.64±0.25)mg·L-1和高浓度氨氮(34.87±0.46)mg·L-1中72h,于胁迫后1、3、6、12、24、48、72 h测定血淋巴和肝胰腺中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GPx)、丙二醛(MDA)含量以及GPx基因相对表达量,结果显示:除低浓度氨氮组外,氨氮胁迫6～24 h,脊尾白虾血淋巴和肝胰腺组织SOD、CAT活力都有不同程度的升高,胁迫48～72 h上述指标则均被抑制;氨氮胁迫过程中血淋巴GPx活力和GPx基因表达显著高于对照组(P<0.05),而肝胰腺中GPx基因表达在48～72 h有所下降;血淋巴和肝胰腺中MDA含量随着胁迫时间的延长呈现上升的趋势,且上述指标在肝胰腺中的活力较血淋巴高。实验结果表明,氨氮胁迫3～24 h对脊尾白虾SOD和CAT活力具有一定的诱导作用,但胁迫48～72 h则抑制其活性;脂质过氧化产物MDA含量在整个胁迫过程中有增加的趋势;肝胰腺可能是脊尾白虾主要的氨氮代谢中心。
Ammonia is a common toxic substance in the aquaculture environment.However,there is little information about the effects of ammonia on antioxidant system and lipid peroxidation in ridgetail white prawn Exopalaemon carinicauda.In this study,the prawns were exposed to different concentrations of ammonia（4.79±0.12）mg·L^-1,（9.05±0.18）mg·L^-1,（17.64±0.25）mg·L^-1,and（34.87±0.46）mg·L^-1 for up to 72 h.Activities of superoxide dismutase（SOD）,catalase（CAT）,and glutathione peroxidase（GPx）,malondialdehyde（MDA）content,and GPx gene expression were assessed in haemocytes and hepatopancreas at 1,3,6,12,24,48 and 72 h after ammonia stresses.In all ammonia stresses except low concentration,activities of SOD and CAT in haemocytes and hepatopancreas increased between 6h to 24 h after ammonia stresses,but reduced at 48 h and 72 h,as compared with the control.The GPx activity and GPx gene expression in haemocytes were significantly higher in the treatments than in the control（P〈0.05）,but the expression level of GPx gene in hepatopancreas decreased under ammonia stresses.Contents of MDA in haemocytes and hepatopancreas gradually increased with increasing ammonia stresses,with higher MDA in hepatopancreas than in haemocytes.The results also showed that SOD and CAT in E.carinicauda were induced at 3～24 h,but inhibited at 48 ～72 h after ammonia stresses and that lipid peroxidative product MDA increased under ammonia stresses. The hepatopancreas might be a sensitive tissue to ammonia stress.
出处
《农业环境科学学报》
CAS
CSCD
北大核心
2014年第4期647-655,共9页
Journal of Agro-Environment Science
基金
国家高技术研究发展计划(2012AA10A409)
国家虾产业技术体系专项(CARS-47)
公益性行业(农业)科研专项(201103034)
中国水产科学研究院基本科研业务费(2013A0701)
关键词
脊尾白虾
氨氮胁迫
超氧化物歧化酶
过氧化氢酶
谷胱甘肽过氧化物酶
丙二醛
Exopalaemon carinicauda
ammonia stress
superoxide dismutase（SOD）
catalase（CAT）
glutathione peroxidase（GPx）
malonaldehyde（MDA）
