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高效液相色谱法测定结石通胶囊中绿原酸含量 被引量:4

Determination of Chlorogenic Acid in Jieshitong Capsules by HPLC
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摘要 目的建立高效液相色谱法(HPLC)测定结石通胶囊中绿原酸含量的方法。方法采用C18柱(250mm×4.6mm,5um),乙腈.0.5%磷酸溶液(7:93)为流动相,柱温35℃,流速1.0mL/min,检测波长326nm。结果绿原酸在0.02042~1.0210μg范围内,峰面积与进样量线性关系良好,r=9999,平均回收率为99.00%,RSD2.54%(n=9)。结论该方法重复性、准确性良好,可用于测定结石通胶囊中绿原酸含量。 Objective To establish a HPLC method for determination of chlorogenic acid in Jieshitong Capsules. Methods C18 column (250 minX4.6 mm, 5μm) was used at 35 C with acetonitrile-0.5 % phosphoric acid solution (7:93) as the mobile phase. The flow rate was 1.0 mL/min and the detection wavelength was 326 nm. Results The calibration curve of chlorogenic acid was good linear in the range of 0.02042-1.0210 μg (r=0.9999), and the average recovery was 99.00 % with RSD of 2.54 % (n=9). Conclusion This method is accurate and repeatable. It can be applied to quality control of Jieshitong Capsules.
出处 《食品与药品》 CAS 2014年第4期279-282,共4页 Food and Drug
关键词 结石通胶囊 绿原酸 高效液相色谱法 Jieshitong Capsule chlorogenic acid HPLC
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