摘要
采用PCR-DGGE技术,利用盆栽大豆试验研究施用污泥堆肥对氯嘧磺隆污染土壤真菌群落结构的影响。结果表明:施用污泥堆肥(2%、4%、8%)的土壤中真菌群落多样性指数高于对照,且大豆生长不同时期(播种期、开花期、结荚期和收获期)土壤真菌群落结构差异显著,即开花期和结荚期真菌多样性指数显著高于播种期和收获期。当污泥堆肥施用量为4%时,真菌群落多样性指数最大。同时,施用污泥堆肥有利于促进土壤中氯嘧磺隆的降解。根据真菌18S rDNA的片段序列分析,施用污泥堆肥土壤中真菌可分为4类:被孢霉科(Mortierellaceae)、酵母菌科(Saccharomycetaceae)、牛肝菌科(Boletaceae)和肉盘菌科(Sarcosomataceae),其中酵母菌科(Saccharomycetaceae)是与氯嘧磺隆降解相关的类群,被孢霉科(Mortierellaceae)是土壤有机质和养分含量丰富的标志类群。
Applying sewage sludge compost to soils may promote degradation of organic pollutants via enhancing microbial activities. A pot experiment was carried out to examine the effects of sewage sludge compost applications on chlorimuron-ethyl residue and fungal communi- ty structures in soil. Compared with the control, fungal diversity indexes were increased by sewage sludge compost（2%, 4%, 8%）, with the maximum effects found at 4% compost. Soil fungal community structures were significant different at different growth stages of soybean（sow- ing, flowering, pod and harvest）, with greater diversity indexes at flowering and pod period than at sowing and harvest stages. Applying sewage sludge compost facilitated degradation of Chlorimuron-ethyl in the soil. Based on sequencing of fungal 18S rDNA fragments, the main fungal populations in the soil were MortiereUaceae, Saccharomycetaceae, Boletaceae and Sarcosomataceae. Saccharomycetaceae was mainly related to chlorimuron-ethyl degradation, and Mortierellaceae was regarded as an indicator of rich organic matter and nutrients in soil.
出处
《农业环境科学学报》
CAS
CSCD
北大核心
2014年第3期495-501,共7页
Journal of Agro-Environment Science
基金
国家自然科学基金(31272484
31372351)
