摘要
目的通过病例对照的方式研究缺血性卒中急性期血浆神经递质水平与正常人群之间的差异,同时研究不同严重程度卒中患者的血浆递质水平的差异。方法选取连续入组、发病在14 d内的急性缺血性卒中患者,采集急性期的血样标本,通过液相色谱-高分辨质谱外标法测量比较卒中组以及正常对照组血浆内的5-羟色胺、去甲肾上腺素、谷氨酸和γ-氨基丁酸4种神经递质的浓度。同时比较不同卒中严重程度患者的4种神经递质浓度。结果共268例缺血性卒中患者入组,其急性期血浆神经递质与正常对照组相比,血浆5-羟色胺(t=2.289,P=0.023)、去甲肾上腺素(t=0.0903,P=0.367)和谷氨酸有上升趋势(t=0.277,P=0.782),γ-氨基丁酸有下降趋势(t=-4.4145,P=0.000),其中5-羟色胺和γ-氨基丁酸在两组之间的差异有显著性。中重度卒中患者血浆5-羟色胺水平显著高于轻度卒中患者(t=2.197,P=0.029)。结论缺血性卒中急性期血浆神经递质水平与正常对照相比有差异,中重度卒中患者的血浆5-羟色胺水平升高较轻度卒中患者显著。
Objective To explore the differences in plasma neurotransmitter levels between acute ischemic stroke patients and normal controls, and to investigate the role of neurotransmitters in the stroke severity. Methods Consecutively recruited and less than 14 d acute ischemic stroke patients were selected. Blood samples were harvested from these subjects at the acute stage of stroke. The plasma levels of four neurotransmitters （i.e. 5-hydroxytryptamine, norepinephrine, glutamate, andγ-aminobutyric acid） were determined by using liquid chromatography-high-resolution mass spectrometry in the patients and normal controls. The neurotransmitter levels were also compared between the different stroke severity groups. Results A total of 268 patients enrolled in our study. Compared with the normal controls, the patients with acute ischemic stroke had signiifcantly higher level of 5-hydroxytryptamine （t=2.289, P=0.023） and lower level ofγ-aminobutyric acid （t=-4.4145, P=0.000）;however, there were no significant differences in the levels of norepinephrine （t=0.0903, P=0.367） and glutamate （t=0.277, P=0.782） between these two groups. Compared with the mild stroke severity patients, the patients with moderate or severe stroke had a signiifcantly higher level of 5-hydroxytryptamine （t=2.197, P=0.029）. Conclusion The plasma neurotransmitter levels differ between acute ischemic stroke patients and normal controls. Increased concentration of 5-hydroxytryptamine is correlated with stroke severity.
出处
《中国卒中杂志》
2014年第7期578-582,共5页
Chinese Journal of Stroke
