摘要
目的:研究白芷的活血化瘀活性成分。方法:对白芷药材进行系统化学部位分离得到白芷石油醚、乙酸乙酯、正丁醇、水提部位。大鼠随机分成7组,生理盐水对照组,血瘀模型组,阳性药对照组(阿司匹林12 mg·kg-1)及白芷4个不同提取部位组,每组8只。每组均灌胃给药,白芷不同提取部位剂量20 g·kg-1(相当于原生药),连续给药7 d,每日1次。第7天药后1 h除生理盐水对照组外,其余各组均皮下注射0.1%肾上腺素0.8 mg·kg-1,共2次,间隔4 h,考察白芷各不同提取部位的活血作用。针对活性最强的部位建立10批不同产地样品的HPLC指纹图谱,并对10批不同产地白芷乙酸乙酯部位活血化瘀作用进行检测,利用多元回归和灰色关联度结合方法研究谱效相关性。结果:与正常组相比,模型组全血黏度、血浆黏度、红细胞压积、红细胞聚集指数均明显增高(P<0.01)。与模型组相比,白芷各提取部位组血液流变学指标均有不同程度的改善(P<0.05,P<0.01),其中以白芷乙酸乙酯部位作用最强(P<0.01)。将10批白芷乙酸乙酯部位HPLC指纹图谱按照中位数法建立各样品的对照指纹图谱,共16个共有峰。谱效相关性研究发现4,9,11,12,16号峰是与活血化瘀作用相关色谱峰。结论:4,9,11,12,16峰可能为白芷活血化瘀作用的特征峰,所建立的白芷HPLC特征图谱与药效作用的关系,可为建立"指纹图谱与药效关联"的中药质量控制模式提供理论依据和数据支持。
Objective：To study the active components in Angelicae Dahuricae Radix with activating blood circulation effect.Method：The different extracts from Angelicae Dahurica Radix were obtained by the means of systematic solvent extraction.Rats were randomly divided into 7 groups：control group,model group,positive control group （aspirin 12 mg ·kg-1） and petroleum ether,ethyl acetate,butanol,water extract group （20 g ·kg-1 equivalent to the primary drug）.The drugs were ig given for 7 days,once each day.1 h after the last administration,the rats were injected with 0.1％ epinephrine 0.8 mg · kg-1 with 2 times and 4 h interval to establish the blood stasis model.Then the activating blood circulation activity was studied by hemorheology test.The HPLC fingerprints of 10 different areas of the most active extracts from Angelicae Dahurica Radix were established and the activating blood circulation activity of 10 batches was detected.The spectrum-activity relationship of the extracts was studied by mathematical statistics method.Result：Compared with the normal group,the whole blood viscosity,plasma viscosity,hematocrit,erythrocyte aggregation index were significantly increased in model group （P ＜ 0.01）.Compared with the model group,the hemorrheological indexes in the groups of different extracts of Angelicae Dahurica Radix were improved （P ＜ 0.05-P ＜ 0.01）.The Ethyl acetate extracts had the best activating blood circulation effect （P ＜ 0.01）.The spectrum-activity relationship of the extracts was found that peaks 4,9,11,12,16 of the extracts had the stronger activating blood circulation effect.Conclusion：Common peaks 4,9,11,12,16 could be characteristic peak for activating blood circulation activity of Angelicae Dahurica Radix.The spectrum-activity relationship of Angelicae Dahurica Radix established could provide the theoretical basis.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第15期139-143,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金青年基金项目(81202916)
关键词
白芷
活血化瘀
指纹图谱
谱效关系
Angelicae Dahurica Radix
activating blood circulation
fingerprint
spectrum-activity relationship
