摘要
目的筛检合肥地区无偿献血者的不规则抗体,通过对不规则抗体筛检阳性标本进行鉴定,分析本地区无偿献血者不规则抗体的分布特点并探讨不规则抗体筛检对输血安全的必要性。方法对本地区的每份无偿献血标本分别用凝聚胺法,抗球蛋白法进行不规则抗体筛检,阳性标本用商品化谱细胞试剂采用盐水法,抗球蛋白法进行抗体鉴定及效价检测。结果 2007年1月至2012年12月开展献血者不规则抗体检测项目,共筛检献血者标本343 777份,不规则抗体阳性24例;阳性标本中鉴定出抗-M:13例、抗-D:3例、抗-E:6例、抗-s:1例、抗-N:1例;其中IgM抗体为17例,IgG抗体为6例,IgG抗体+IgM抗体为1例,女性献血者中不规则抗体阳性率远高于男性献血者;抗体效价1∶32的6例,其余18例效价均在1∶2至1∶16之间。结论通过筛检和鉴定,统计得出本地区无偿献血者中不规则抗体多为MNS系统抗体,其次为Rh系统抗体;筛检献血者标本中不规则抗体,对保证输血安全,降低和避免输血反应的发生有着重要的意义。
Objective To screen irregular antibodies of blood donors in Hefei area,to analyze the distribution characteristics of irregular antibodies of blood donors by identifying the positive samples and to investigate the necessity of irregular antibodies screening for the safety of blood transfusions. Methods Blood donors＇ samples from Heifei were screened for irregular antibodies using manual polybrene test and coombs test. The antibodies were identified in positive samples and the antibody titers were measured by saline water test and coombs test with commercialized panel ceils. Results After screening 343 777 donors＇ samples from 2006 to now in Anhui Blood Center, we identified 24 positive samples including anti-M （13 sampies） ,anti-D（3 samples） ,anti-E（6 samples） ,anti-s（ 1 sample） and anti-N（ 1 sample）. There were IgM antibodies（ 17 sampies）, IgG antibodies（6 samples） and IgM ＋ IgG antibodies （1 samples）. The positive rate of irregular antibodies of female donors are much higher than the male donors. There were 6 samples with the antibody titer 1： 32 while the titers of other samples were between 1：2 and 1： 16. Conclusion The major antibodies are MNS system antibodies, followed by Rh system antibodies in our local area. Screening irregular antibodies in donors＇ sample has great significance in ensuring the safety of blood transfusion and reducing and preventing transfusion reactions.
出处
《中国输血杂志》
CAS
CSCD
北大核心
2014年第7期709-711,共3页
Chinese Journal of Blood Transfusion
关键词
献血者
不规则抗体筛检
抗体鉴定
抗体效价
blood donor
irregular antibody screening
antibody identification
antibody titer
