摘要
目的研究重复测试和不同性别两个因素对小鼠在旷场试验中自主行为活动和焦虑行为的影响。方法通过动物视频行为分析系统,分别记录和分析雌雄小鼠5min内在旷场中的总路程、总体平均速度、总体平行指数、中央区的路程占总路程的比例、中央区的平均速度、中央区进入次数、中央区停留时间占整体测试时间的比例和中央区平行指数,每天测试1次,共测试3d。结果在旷场试验中,小鼠的总路程、总的平均速度、总的平行指数、中央区路程占总路程的比例、中央区的平均速度、进入中央区次数、中央区停留时间和中央区的平行指数,随测试次数的增加呈下降趋势(均为P<0.001);整体而言,以上指标在雌雄小鼠之间无显著性差异(均为P>0.05),但随测试次数增加,雄性小鼠的下降趋势似乎比雌性小鼠明显,尤其在第三次测试中,雄性小鼠在中央区的平均速度(P<0.05)和平行指数(P<0.01)比雌性小鼠更低。结论重复测试旷场试验会影响小鼠的自主行为活动,同时也会干扰焦虑状态的评价;不同性别对小鼠的初次旷场试验测试无影响,但重复测试对雄性小鼠的自主行为活动的影响似乎比雌性小鼠更大。
OBJECTIVE To study the effect on the locomotory activity and anxiety-like behavior of laboratory mice by both factors of repeated measurement and sex. METHODS The total distance traveled, total mean velocity, total parallel index, the percentage of distance in center zone, the mean velocity in center zone, the center zone entries, the percentage of time spent in center zone and the parallel index in center zone of the female and male mice for 5 minutes were recorded and analyzed in opert, field test by animal video tracking analysis system, once a day for 3 days. RESULTS In the open field test, the indexes of the total distance traveled, total mean velocity, total parallel index, the percentage of distance in center zone, the mean velocity in center zone, the center zone entries, the percent- age of time spent in center zone and the parallel index in center zone in mice showed the downtrend as the measurement numbers were increasing （P 〈 0. 001 ）. In general,the sex did not make the indexes mentioned above signifi- candy different （ P 〉 0. 05 ）. However, as repeating the tests, the downtrend of these in male mice were seemly more significant than female mice, especially in the third time measurement, in which the mean velocity （P 〈 0. 05 ） and parallel index （P 〈 0.01 ） in center zone were lower than the ones in female mice, respectively. CONCLUSION The repeated measurement inferences the locomotory activity and interrupted the anxiety state of mice. Sex of mice does not make the behavior in open field different at the first time, but the repeated measurement seemly more significandy affect the locomotory activity of the male mice than the female.
出处
《海峡药学》
2014年第7期49-51,共3页
Strait Pharmaceutical Journal
基金
国家自然基金青年科学基金项目(81302756)
教育部高等学校博士学科点专项科研基金(20133518110004)
福建省自然科学基金青年科技人才创新项目(2013J05118)
福建医科大学博士启动基金(2011bs003)
关键词
小鼠
旷场试验
自主行为活动
焦虑行为
Mice
Open field test
Locomotor activity
Anxiety-like behavior
