摘要
目的 观察卡介苗 (bacilluscalmetteguerin ,BCG)雾化吸入应用于呼吸道的可行性及对呼吸道动物过敏性疾病的免疫调节作用 ,并探讨作用机理。方法 将成年大鼠分为盐水对照组、卵清蛋白致敏组、BCG雾化吸入组和BCG雾化吸入 +卵清蛋白致敏组。对各组动物做行为学观察 ,鼻黏膜及气管组织学检查 ,支气管肺泡灌洗并涂片 ,反转录多聚酶链反应 (reversetranscriptasepolymerasechainreaction ,RT PCR)法检查各组肺组织干扰素 γ(interferon γ ,IFN γ)及白介素 4 (interleukin 4 ,IL 4 )的mRNA水平 ,酶联免疫吸附测定 (enzyme linkedimmunosorbentassay ,ELISA)法检测各组血清及支气管肺泡灌洗液上清中IL 4及IFN γ的蛋白水平。结果 卵清蛋白致敏组动物模型致敏成功 ;BCG雾化吸入组动物行为无明显异常 ,鼻及气管黏膜有少许炎细胞浸润 ,支气管肺泡灌洗液涂片巨噬细胞明显增多 ,IFN γmRNA及蛋白明显增多 ;BCG雾化 +致敏组过敏症状明显减轻 ,鼻黏膜及支气管肺泡灌洗液涂片变应性炎症轻微 ,IFN γmRNA及蛋白增多 ,IL 4mRNA及蛋白明显减少。结论 动物实验证实BCG呼吸道雾化给药是一可以考虑的给药途径 ,可有效形成局部免疫 ,对呼吸道变应性疾病具有显著的抑制效应 。
Objective To investigate whether bacillus calmette guerin (BCG) is effective used transairway and its preventive mechanisms to allergic airway diseases Methods Animal experiment was finished Adult rats were devided into four groups: control group, ovalbumin sensitized group, BCG used transairway group, BCG used transairway + ovalbumin sensitized group Then these animals symptoms were studied and the pathology change were studied under microscope about nasal and bronchi mucosa and bronchoalveolar lavege fluid cells IL 4 mRNA and IFN γ mRNA in lung tissue were detected through RT PCR, the protein production of IL 4 and IFN γ about bronchoalveolar lavege fluid and serum were detected through ELISA Results In ovalbumin sensitized group, allergic animal model were made successfully In only BCG used transairway group,the symptoms of animals were normal, few inflammation cells infiltrated into the mucosa of nasal and bronchi, the numbers of macrophage were greatly increased in smear of bronchoalveolar lavege fluid, IFN γ mRNA and protein production were greatly increased In BCG used transairway + ovalbumin sensitized group, the allergic symptoms and inflammation were greatly reduced, not only IFN γ mRNA and protein production were increased but also IL 4 mRNA and protein production were greatly decreased. Conclusion It is a good pathway that BCG used transairway. The immunoloregulation mechanisms of BCG to allergic airway diseases are to enhance Th1 response, in the meantime, to suppress Th2 response.
出处
《中华耳鼻咽喉科杂志》
CAS
CSCD
北大核心
2002年第4期267-270,T003,共5页
Chinese Journal of Otorhinolaryngology
