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逆灸关元、命门穴对极限耐力运动大鼠NOS、钠-钾-ATP酶活性的影响 被引量:3

Effect of Preventive Moxibustion at Guanyuan(CV-4) or Mingmen(DU-4) on NOS and Na-K-ATPase Activity of Extreme Endurance Exercise Rats
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摘要 目的:观察逆灸关元、命门穴对大鼠极限运动时的运动耐力及心肌一氧化氮合酶(NOS)、骨骼肌及心肌钠-钾-ATP酶活性的影响。方法:将大鼠按游泳能力随机分为正常对照组、力竭对照组、逆灸关元组、逆灸命门组、逆灸关元力竭组、逆灸命门力竭组,分别干预20 d后进行力竭游泳,观察力竭时间并检测相应的酶活性。结果:与正常组比较,力竭组钠-钾-ATP酶活性明显降低,NOS活性明显升高(P<0.05,P<0.01);而逆灸关元、命门对酶活性影响不大(P>0.05)。与力竭组比较,逆灸关元、命门均可使力竭大鼠游泳时间延长,钠-钾-ATP酶活性增高(P<0.05,P<0.01);逆灸命门还使其NOS活性明显降低且与逆灸关元组比较有统计学意义(P<0.01,P<0.05)。结论:逆灸命门、关元穴均能提高极限运动大鼠的运动耐力,延长其游泳力竭时间,其机制可能与其调节NOS、激发钠-钾-ATP酶的活性有关。 Objective:To observe the effect of preventive moxibustion at Guanyuan(CV-4),Mingmen(DU-4)on exercise tolerance and myocardium NOS,skeletal and cardiac muscle Na-K-ATPase activity of extreme exercise rats. Methods:Rats were randomly divided into normal control group,exhaustion control group,Guanyuan moxibustion group,Mingmen moxibustion group,Guanyuan moxibustion exhaustion group,Mingmen moxibustion exhaustion group according to their swimming exhausted time. Rats received an exhaustive swimming after 20 days of intervention. Exhausted time of swimming rats were recorded. The NOS and Na-K-ATPase activity of rats were detected. Results:Compared with the normal group,the exhaustion group Na-K-ATPase activity decreased significantly and NOS activity was significantly increased(P〈0.05,P〈0.01).Preventive moxibustion at Guanyuan(CV-4) or Mingmen(DU-4) had little effect on the enzyme activity(P0.05). Compared with the exhaustion group,preventive moxibustion at Guanyuan(CV-4) or Mingmen(DU-4)significantly increased swimming time of exhausted rats and Na-K-ATPase activity also increased significantly(P〈0.05,P〈0.01),preventive moxibustion Mingmen(DU-4) was significantly decreased NOS activity and there were significant differences between preventive moxibustion at Guanyuan(CV-4)(P〈0.05,P〈0.01).Conclusion:Preventive moxibustion at both Mingmen(DU-4) and Guanyuan(CV-4) could improve the exercise tolerance of rats and prolong their swimming exhausted time. The mechanism may be adjusted NOS and excited Na-K-ATPase activity.
出处 《山东中医药大学学报》 2014年第4期367-369,共3页 Journal of Shandong University of Traditional Chinese Medicine
基金 国家自然科学基金课题(编号:90209026)
关键词 逆灸 极限耐力运动 一氧化氮合酶 钠-钾-ATP酶 关元 命门 大鼠 preventive moxibustion extreme endurance exercise NOS Na-K-ATPase Guanyuan Mingmen rat
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