摘要
目的:研究AKT和mTOR在子宫内膜异位症(EMS)患者的异位内膜及正常子宫内膜中的表达,探讨其与子宫内膜异位症发生、发展的关系。方法:采用免疫组织化学技术检测p-AKT及p-mTOR的蛋白表达,应用RT-PCR检测AKT及mTOR的mRNA表达水平。结果:免疫组化结果显示异位内膜组p-AKT、p-mTOR阳性表达率(92.5%,87.5%)明显高于正常子宫内膜组(62.5%,47.5%),两组比较,通过卡方检验差异有统计学意义(χ2=10.32,14.59,P均<0.01)。RTPCR检测结果显示异位内膜组AKT、mTOR的相对表达量(0.742 6±0.112 2,0.717 2±0.131 8)明显高于正常子宫内膜组(0.456 9±0.095 9、0.324 8±0.042 0),两组比较,通过q检验差异有统计学意义(q=9.52,14.53,P均<0.05)。结论:PI3K/AKT/mTOR信号通路参与子宫内膜异位症的发生、发展过程,其调节异常可能在子宫内膜异位症患者的异位灶形成过程中发挥促进作用。
Objective： To research the expression levels of AKT and mTOR in ectopic endometrium of patients with endometriosis and normal endometrium of patients without endometriosis, explore their relationships with occurrence and development of endometriosis. Methods： Immunohistochemical assay was used to detect the expression levels of p - AKT protein and p - roTOR protein, RT - PCR was used to detect the expression levels of AKT mRNA and mTOR mRNA. Results ： Immunohistochemical assay results showed that the positive expression rates of p -AKT protein and p -mTOR protein in ectopic endometrium group were 92. 5% and 87.5%, respectively, which were statistically significantly higher than those in normal endometrium （62. 5% and 47. 5%, respectively） （χ2 = 10. 32, P 〈01 01 ; χ2 = 14. 59, P 〈0. 01 ） . RT- PCR results showed that the relative expression levels of AKT mRNA and mTOR mRNA in ectopic endometrium group were （0. 742 6 ±0. 112 2） and （0. 717 2 ±0. 131 8）, respectively, which were statistically significantly, higher than those in normal endometrium [ （0. 456 9 ± 0. 095 9） and （0. 324 8 ± 0. 042 0）, respectively ] （ q = 9. 52, P 〈 0. 01 ; q = 14. 53, P 〈 0. 05 ） . Conclusion： PI3K/Akt/mTOR signal pathway is involved in occurrence and development of endometriosis, and its dysregulation may play a promoting role in formation of ectopic foci in patients with endometriosis.
出处
《中国妇幼保健》
CAS
北大核心
2014年第23期3826-3828,共3页
Maternal and Child Health Care of China
