摘要
目的：探讨丹参多酚酸盐对心衰模型大鼠去甲肾上腺素（noradrenaline，NE）、血管紧张素-Ⅱ（angiotension-Ⅱ，Ang-Ⅱ）、肿瘤坏死因子-α（tumor necrosis factor-a，TNF-a）的影响。方法60只雄性SD大鼠采用随机数字表法分为6组各10只，即正常对照组、模型组、卡托普利组、丹参多酚酸盐低剂量、丹参多酚酸盐高剂量组、卡托普利联合丹参高剂量组。除正常对照组，其余5组大鼠腹腔注射阿霉素制造心衰模型。造模的同时，各组开始相关干预。8周后，检测各组大鼠的左室收缩压（left ventricular systolic pressure，LVSP）、左室舒张末压（left ventricular end-diastolic pressure，LVDP）、左室内压最大上升速度（＋dp/dtmax）、左室内压最大下降速度（-dp/dtmax），血清NE、Ang-Ⅱ、TNF-α水平。Western-blot方法检测心肌Ang-Ⅱ、TNF-α的表达。结果与模型组LVSP、LVDP、＋dp/dtmax、-dp/dtmax [分别为（90.77±14.75）mmHg、（22.52±2.58）mmHg、（3290.16±109.61）mmHg/s、（3114.07±112.39）mmHg/s]比较，丹参多酚酸盐高剂量组[分别为（114.10±13.71）mmHg、（19.97±1.14）mmHg、（3504.97±163.94）mmHg/s、（3303.02±121.98）mmHg/s]，卡托普利组[分别为（119.77±15.70）mmHg、（18.88±2.43）mmHg、（3562.78±148.16）mmHg/s、（3372.93±156.13）mmHg/s]，卡托普利联合丹参高剂量组[分别为（141.18±15.42）mmHg、（15.58±1.46）mmHg、（3766.56±159.93）mmHg/s、（3566.70±154.57）mmHg/s]差异均有统计学意义（P＜0.05或0.01），且卡托普利联合丹参高剂量组与卡托普利组比较，差异有统计学意义（P＜0.05）。与模型组血清NE、Ang-Ⅱ、TNF-α水平[（85.06±9.07）ng/ml、（180.11±25.45）pg/ml、（205.80±15.73）pg/ml]比较，丹参多酚酸盐高剂量组[（75.33±8.60）ng/ml、（149.21±25.39）pg/ml、（188.84±13.79）pg/ml]、卡托普利组[（71.49±6.21）ng/ml、（139.15±24.83）pg/ml、（183.73±10.99）pg/ml]、卡托普利联合丹参高剂量组[（60.02±7.38）ng/ml、（110.68±28.63）pg/ml、（165.93±16.22）pg/ml]均有不同程度降低，差异均有统计学意义（P＜0.05或0.01），且卡托普利联合丹参高剂量组低于卡托普利组（P＜0.05）。与模型组心肌 Ang-Ⅱ、TNF-α[（1.043±0.044）、（1.167±0.048）]表达比较，丹参多酚酸盐高剂量组[（0.981±0.024）、（1.069±0.055）]、卡托普利组[（0.954±0.031）、（1.046±0.053）]、卡托普利联合丹参高剂量组[（0.886±0.044）、（0.955±0.038）]均降低（P＜0.05或＜0.01），且卡托普利联合丹参高剂量组低于卡托普利组（P＜0.05）。结论丹参多酚酸盐可降低心衰大鼠血清NE、Ang-Ⅱ、TNF-α水平，减少心肌Ang-Ⅱ、TNF-α表达。
Objective To investigate the effects of salvianolate on noradrenaline（NE）, angiotension-Ⅱ（Ang-Ⅱ）and tumor necrosis factor-α（TNF-α）in rats with congestive heart failure. Methods Sixty male SD rats were randomly divided into 6 groups, the normal control group（NCG）, the model group, the captopril group（CAG）, the low dosage of salvianolate group（LSG）, the high dosage of salvianolate group（HSG）, the captopril and high doseage of salvianolate group（CSG）. The rats of Congestive Heart Failure were established＆amp;nbsp;with peritoneal injection of adriamycin except the rats in normal control group. The rats in normal control group were injected with the equal volume of normal saline once per week for 6 weeks. The medication was started in every group at the same time. Eight weeks later, the Left Ventricular Systolic Pressure（LVSP）, Left Ventricular End-diastolic Pressure（LVDP）, ＋dp/dtmax, -dp/dtmax were measured. The serum levels of NE, Ang-Ⅱ and TNF-αwere measured. Protein expressions of Ang-Ⅱ and TNF-αin cardiac muscle were detected by Western blot. Results Compared with the model group[（90.77±14.75）mmHg, （22.52±2.58）mmHg, （3 290.16± 109.61）mmHg/s, （3 114.07±112.39）mmHg/s], HSG[（114.10±13.71）mmHg, （19.97±1.14）mmHg, （3 504.97 ± 163.94）mmHg/s, （3 303.02 ± 121.98）mmHg/s ] and CSG [ （141.18 ± 15.42）mmHg, （15.58 ± 1.46）mmHg, （3 766.56±159.93）mmHg/s, （3 566.70±154.57）mmHg/s]had significant difference in LVSP, LVDP, ＋dp/dtmax, -dp/dtmax（P〈0.05 or 0.01）. Compared with the model group[（85.06±9.07）ng/ml, （180.11±25.45）pg/ml, （205.80±15.73）pg/ml], the serum levels of NE, Ang-Ⅱand TNF-αwere decreased in groups of HSG[（75.33±8.60）ng/ml, （149.21±25.39）pg/ml, （188.84±13.79）pg/ml], CAG[（71.49± 6.21）ng/ml, （139.15 ± 24.83）pg/ml, （183.73 ± 10.99）pg/ml ] and CSG [ （60.02 ± 7.38）ng/m, （110.68 ± 28.63）pg/ml, （165.93±16.22）pg/ml]in different degree（P〈0.05 or 0.01）, those of CSG were significantly lower than CAG（P〈0.05）. Compared with the model group[（1.043±0.044）, （1.167±0.048）], the expression of protein in Ang-Ⅱand TNF-α were decreased in groups of HSG[（0.981±0.024）, （1.069±0.055）]CAG [（0.954±0.031）, （1.046±0.053）]and CSG[（0.886±0.044）, （0.955±0.038）]in different degree（P〈0.05 or 0.01）, those of CSG were significantly lower than CAG（P〈0.05）. Conclusion Salvianolate can reduce the serum levels of NE, Ang-Ⅱand TNF-αin rats with congestive heart failure, decrease the expression of Ang-Ⅱand TNF-αin its cardiac muscle, improve the cardiac function.
出处
《国际中医中药杂志》
2014年第8期702-706,共5页
International Journal of Traditional Chinese Medicine
基金
基金项目：2011年福建省卫生厅青年科研课题（项目编号：2011-1-41）
2013年福建省中青年教师教育科研项目（项目编号：JB13105）
