摘要
将24头产后7 d的荷斯坦奶牛随机分为4组,高、中、低剂量免疫组(记为T1、T2、T3)分别注射1010、109、108 CFU/mL无抗性基因的抑制素真核表达质粒基因疫苗(重组菌C500(pXAIS))各3 mL,对照组(CK)注射10%生理盐水3 mL。初次免疫28 d后进行加强免疫。用B超诊断仪检测产后奶牛子宫恢复情况。结果表明:加强免疫能提高抗抑制素抗体P/N值,各免疫组P/N值均高于对照组,其中T1和T2组均与对照组差异显著(P<0.05),加强免疫7 d,后T1组抗体阳性率最高,达83.33%;抑制素基因疫苗免疫30 d内,前21 d子宫颈和子宫孕角恢复较快,后7 d恢复较慢,各免疫组奶牛产后子宫颈恢复时间短于对照组,但差异不显著(P>0.05);各免疫组产后子宫孕角恢复时间比对照组短,且T1组的恢复时间(28.75±1.92)d)与对照组((33.75±1.09)d)差异显著(P<0.05)。以上结果表明,在本试验条件下,非抗性筛选的抑制素真核表达质粒基因疫苗免疫产后奶牛,加强免疫后能引起较好的免疫应答,且能促进奶牛产后子宫复旧。
Twenty four dairy cows at 7 d after calving were randomly assigned to four groups（three immunized group and one control group）, the three immunized group of cows were primarily immunized（injection day as 0 d） with 3 mL（per cattle cow） of recombinant bacterium C500（pXAIS） delivering the inhibin gene vaccine（a kind of DNA vaccine without antibiotic resistance gene） in a high（1010 CFU/mL）, middle（109 CFU/mL） or low（108 CFU/mL） dosage, the control group of cows were intramuscularly immunized with physiological saline（3 mL per cattle cow）. The booster immunization（the same dosage as the primary immunization） was performed 28 d after primary immunization. The B ultrasonography was used to investigate the effect of inhibin gene vaccine on antibody level, involution of uterine cervix and uterine gravid horn of the postpartum dairy cows. The result showed that the booster immunization could raise antibody level against inhibin, which in T1 and T2 group was higher than that in CK（P〈0.05）. Furthermore, positive antibody percentage 7 d after booster immunization in T3 group was the highest, which was 83.33%. Diameters of both postpartum uterine cervix and postpartum gravid uterine horn in all groups decreased sharply during the first 21 d and then decreased gradually during the final 7 d within 30 d after inhibin gene vaccine immunization. Days of the uterine cervix involution were shorter（P〉0.05） in the three immunized groups than those in control group, the same as those of the gravid uterine horn involution except for the remarkable difference（P〈0.05） between T1 group（（28.75±1.92） d） and the CK group（（33.75±1.09） d）. These findings demonstrate that inhibin gene vaccine C500（pXAIS） can enhance the immune response, stimulate restoration of uterus of the postpartum dairy cows.
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2014年第4期395-398,共4页
Journal of Hunan Agricultural University(Natural Sciences)
基金
国家自然科学基金项目(31101774)
中国博士后科学基金特别资助项目(2012T50220)
现代农业产业技术体系项目(CARS–37–04B)
关键词
奶牛
抑制素
基因免疫
子宫
dariy cow
inhibin
gene immunization
uterus
