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Livin基因在结直肠癌中的表达及其与PTEN和Caspase-3的相关性研究 被引量：7

Expression of Livin gene in colon cancer and its relationship with PTEN and Caspase- 3
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摘要 目的探讨Livin基因在结直肠癌中的表达及其与PTEN和Caspase-3的相关性。方法收集结直肠癌、腺瘤及大肠正常黏膜组织各50例,应用免疫组织化学SP染色方法检测Livin、PTEN及Caspase-3的表达情况并进行评分。结果结直肠癌、腺瘤及大肠正常黏膜组织中Livin蛋白的阳性表达率分别为64.00%、56.00%、18.00%,结直肠癌组织中Livin蛋白的阳性表达率高于腺瘤及大肠正常黏膜组织;Livin蛋白阳性表达率与淋巴结转移及Duke分期有关,而与结直肠癌患者的年龄、性别、分化程度无关。在结直肠癌、腺瘤及大肠正常黏膜组织中PTEN蛋白的阳性表达率分别为40.00%、76.00%、98.00%,Caspase-3蛋白的阳性表达率分别为46.00%、84.00%、92.00%,结直肠癌组织中PTEN、Caspase-3蛋白的阳性表达率明显低于腺瘤及正常黏膜组织,差异有显著性意义(P<0.01);PTEN、Caspase-3阳性表达率与结直肠癌Duke分期及淋巴结转移密切相关,与结直肠癌患者的年龄、性别及肿瘤分化程度无关。在结直肠癌组织中Livin蛋白阳性表达与PTEN及Caspase-3蛋白表达均呈负相关。结论凋亡抑制因子Livin、凋亡因子Caspase-3及抑癌基因PTEN参与了结直肠癌的发生、发展。在结直肠癌中PTEN蛋白活性被抑制加速了Livin促进结直肠癌的发生。Livin促进结直肠癌的发生可能通过抑制Caspase-3的活性实现。 Objective To explore Livin,PTEN and Caspase-3 protein expression and their correlationship in colon canc-er.Methods The expressions of Livin ,PTEN and Caspase-3 protein in 50 cases of colon cancer , colon adenoma and nor-mal colorectal mucosa were detectded by immunohistochemistry staining .Results （1）The expression of Livin in colon canc-er, colon adenoma and normal colorectal mucosa was 64.00%,56.00%,18.00%.Livin protein positive expression rates in colon cancer tissues was more than those in colon adenoma and nomal colorectal mucosa and had statistical significance . （2） The expression of PTEN in colon cancer , colon adenoma and normal colorectal mucosa was 40.00%,76.00%, 98.00%.The expression of Caspase -3 in colon cancer, colon adenoma and normal colorectal mucosa was 46.00%, 84.00%, 92.00%.Caspase-3 and PTEN protein positive expression rates in colon cancer tissues were less than those in colon adenoma and nomal colorectal mucosa （P〈0.01）.（3） Caspase-3,PTEN protein expression had prominent inverse correlation with the Livin expression .Conclusion Livin,PTEN,Caspase-3 participate the happen and the development of colon cancer .The suppressed of PTEN protein may accelerate the express of Livin , and promote the happening of colon cancer.Livin promotes colon cancer through inhibited caspase -3 protein activity .
作者 姚文娟 陈素萍 宋会双 张伟强
机构地区 中国石油天然气集团公司中心医院病理科 中国石油天然气集团公司中心医院神经外科
出处 《大连医科大学学报》 CAS 2014年第3期221-224,共4页 Journal of Dalian Medical University
基金 河北省廊坊市科技支撑计划项目(2013013006)
关键词 LIVIN PTEN CASPASE-3 免疫组织化学 colon cancer Livin PTEN Caspase - 3 immunohistochemistry staining
分类号 R735.34 [医药卫生—肿瘤]
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参考文献7

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二级参考文献30

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大连医科大学学报
2014年 第3期

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