摘要
目的 建立单纯性脑震荡（pure cerebral concussion,PCC）和三重脑震荡（multiple cerebral concussion,3MCC）大鼠模型,观察上述2种大鼠损伤后早期中隔冠状断面小胶质细胞的反应变化,探讨小胶质细胞（MG）在不同脑损伤后的病理变化规律.方法 （1）动物分组：成年健康SD大鼠30只,体重（260±20）g,随机分为正常组、PCC和3MCC模型组,各模型组再随机分为1d组、4d组二个观测点,各组含动物6只.（2）采用金属单摆闭合性脑损伤打击装置复制PCC、3MCC大鼠模型,其中PCC大鼠12只,3MCC大鼠12只.（3）采用抗CD11b抗体行免疫组化染色,用显微图像分析系统,对鼠脑中隔冠状断面的额顶叶皮质区（FrPa）、胼胝体（CC）、尾壳核（CPu）、侧脑室旁中央间隔区（MS-LV）等脑区的CD11b阳性细胞数进行观测分析.结果 （1）正常状态下,CD11b阳性细胞很少; （2） PCC、3MCC模型组中隔断面上述各脑区的CD11b阳性细胞数在伤后明显增加,与正常组比较差异有统计学意义（P＜0.05）; （3） 3MCC组CD11b阳性细胞数的增加高于PCC组（P＜0.05）.结论 （1） PCC和3MCC伤后1～4 d中隔断面MG出现明显激活,提示MG参与了PCC和3MCC的伤后病理生理改变过程; （2）随损伤次数增加,CD11b阳性细胞数呈明显损伤累积性增加.
Objective To establish a rat model of the Pure Cerebral Concussion （PCC） and Multiple Cerebral Concussion （3MCC）,so as to observe and make comparison of the reactions and changes in microglia in the saeptum coronary section of the early injured rats.Methods （1） Rats grouping：30 healthy adult SD rats with weight of 260 ± 20g,were divided randomly into normal group,PCC model group and 3MCC model group.The model groups were regrouped into 1d group and 4d group for observation.（2） The Metal pendulum closed brain injury blow device was used to set up PCC model and MCC model in order to replicate 12 rats of PCC model and MCC model respectively.（3） The immunohistochemical S-P method was adopted to dye the CD1 1b antibodies.After that,we observed the expressions of rats＇ CD1 1b in FrPa,cc,CPu and MS-LV in the septal coronary denomination parietal lobe.Then we measured and analyzed the reactant of the CD 1 1 b through Image Analysis Technology.Results （1） Under norma1 condition,the expression of the CD11b was rare; （2） The expression of CD11b in different brain areas which were partitioned in PCC mode1 and MCC model increased gradually after injury.And compared with the normal group,they were significantly different （P＜0.05） （3） The expression increase tendency of CD11b in 1d and 4d of MCC group after injure were higher than the 1d and 4d in PCC group.Conclusions （1） MG in the partition surface can be activated in the early injure of PCC and MCC,suggesting that MG is involved in the pathological changes of PCC and MCC after injury; （2） The expression of CD11b increases regularly with the exacerbation of injury severity.
出处
《昆明医科大学学报》
CAS
2014年第8期4-8,共5页
Journal of Kunming Medical University
基金
国家自然科学基金资助项目(81360467)
云南省应用基础研究(联合专项)重点项目(2013FB104)
