摘要
采用正交试验设计方法,以大薯带节茎段为外植体,离体诱导类原球茎并建立大薯类原球茎的再生体系,以解决愈伤组织分化成苗和试管苗移栽成活率低的难题。结果表明:以带节茎段为外植体诱导类原球茎的最适培养基为MS(含3×Ca2+)+1.0 mg·L-1 6-BA+0.2 mg·L-1 NAA+0.1%PVP+3%蔗糖,诱导率高达93.33%;类原球茎增殖的最适培养基为MS+4mg·L-1 6-BA+80 mg·L-1 Ad+0.1%PVP+3%蔗糖;类原球茎生根的最适培养基:1/2MS+0.10 mg·L-1 NAA+0.1%PVP+3%蔗糖。将诱导得到的生根类原球茎植株进行炼苗,移栽基质珍珠岩:蛭石=2:1,移栽成活率可达到95%。
To resolve problem of low survival rate ofDioscorea alata cultured in vitro, orthogonal experimental design method was employed to induce protocorm-like body （PLB） by stem segments with axillary bud of D. alata, and establish regeneration system of PLBs. Results showed： the suitable medium for PLB induction was MS （with 3×Ca2＋）＋1.0 mg·L^-1 6-BA＋0.2mg·L^-1NAA＋0.1% PVP＋3% sucrose, induction rate was 93.33%; the optimum medium for PLB multiplication was MS＋4mg·L^-1 6-BA＋80mg·L^-1 Ad＋0.1% PVP＋3% sucrose; the optimum medium for PLB roots differentiation was 1/2MS＋0.10mg·L^-1 NAA＋0.1% PVP＋3% sucrose. Acclimatized with the ratio of perlite and vermiculite is 2：1, and after transplantation, the survival rate of rooted PLBs plantlets could reach over 95%.
出处
《植物生理学报》
CAS
CSCD
北大核心
2014年第7期1027-1032,共6页
Plant Physiology Journal
基金
海南省重大科技项目(ZDZX2013023)
高等学校博士学科点专项科研基金(20114601110001)
海南大学地方服务项目(HDSF201304)
关键词
大薯
正交试验设计
类原球茎
Dioscorea alata
orthogonal experimental design
PLB （protocorm-like body）
