摘要
目的探讨丹栀逍遥散对广泛性焦虑障碍的作用机制。方法观察丹栀逍遥散对饮水冲突联合天敌暴露应激焦虑模型大鼠行为学的影响。30只大鼠随机分为正常组、模型组、中药组,于造模结束前7 d开始中药干预。除正常组外,其余2组采用饮水冲突法联合天敌暴露法建立应激焦虑大鼠模型,期间2种造模方法交替进行20 d。最后1次应激结束之后,于24 h后记录观察药物干预后,各组大鼠在旷场实验和高架十字迷宫测试结果的异同。结果最后1次复合应激结束24 h之后,相对于正常组,模型组大鼠,水平、垂直运动次数减少,OE%和OT%比例下降;相对于模型组,中药组大鼠,水平、垂直运动次数增加,OE%和OT%比例上升,除OT%外以上结果均有显著差异。结论丹栀逍遥散对于饮水冲突天敌暴露应激焦虑模型大鼠表现有明显抗焦虑作用。
Objective To investigate the effective mechanism of Danzhi Xiaoyao San to generalized anxiety disorder. Methods The influence of Danzhi Xiaoyao San on the ethology in rat model of stress anxiety in Vogel ~s drinking conflict test combining predator exposing test. All 30 rats were randomly divided into normal group, model group and Chinese medicinal group. The rat model of stress anxiety was established by using Vogel^s drinking conflict test combining predator exposing test in model group and Chinese medicinal group for 20 d. After the last time of stress finished and 24-h medicinal intervention, the test results of open field test （OFT） and elevated plus maze （EPM） were compared among all groups. Results After the last time of combined stress for 24 h, the times of horizontal movement and vertical movement, and percentage of open arm entry （ OE% ） and percentage of open arm time （ OT% ） decreased in model group compared with normal group. The times of horizontal movement and vertical movement and OE% and OT% increased in Chinese medicinal group compared with model group. All the above results had significant difference except of OT%. Conclusion Danzhi Xiaoyao San has a significant anti-anxiety effect in rat model of stress anxiety in Vogel＇s drinking conflict test combining predator exposing test.
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2014年第7期486-489,共4页
Journal of Beijing University of Traditional Chinese Medicine
基金
国家自然科学基金项目(No.81173231)
北京中医药大学创新团队项目(No.2011-CXTD-26)
关键词
天敌暴露
饮水冲突
广泛性焦虑障碍
丹栀逍遥散
行为学
大鼠
predator esposing
drinking conflict
generalized anexiety disorder
Danzhi Xiaoyao San
ethology
rats
