摘要
在乙醇-硫酸铵双水相体系中,以苎麻皮为原料,采用微波法提取苎麻皮中的总黄酮。通过单因素实验考察了乙醇浓度、料液比、硫酸铵用量、提取功率、提取时间对提取率的影响。用正交试验确定了最优提取条件,即在料液比1:50 g/mL、硫酸铵用量为0.2 g/mL、提取功率为200 W、提取时间为120 s,乙醇体积浓度为70%时提取率可达2.442%。采用了水杨酸法和邻苯三酚自氧化法对黄酮抗氧化性进行了研究,结果表明苎麻皮黄酮对O-·2和·OH具有较强的清除作用。
In this study, microwave extraction technology integrated with ethanol and aqueous ammonium sulfate solution two-phase separation was applied to extract total flavonoids from Ramie Skin. Based on single factor experiment, ethanol concentration,solid-liquid ratio,the concentration of ammonium sulfate, extraction power and the time of extraction on extraction yield of total flavonoids were investigated. By orthogonal experlment, the optimum extracting conditions were optimized as follows：ratio of solid to liquid was 1：50 g,/mL,the concentration of ammonium sulfate was 0.2 g/mL,extraction power was 200 W,extraction time was 120 s,the ethanol concentration was 70% ,the highest extraction yield reached to 2. 442% under the optimized extraction conditions. In addition ,the total flavonoids extract showed scavenging activities on O2- and ·OH in the antioxidant activity assays.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2014年第8期1299-1302,1320,共5页
Natural Product Research and Development
基金
内江师范学院大学生科研项目(12NSD-2)
四川省教育厅自然科学基金资助重点项目(13ZA0004)
关键词
苎麻皮
双水相
微波
总黄酮
抗氧化性
Ramie skin
microwave
aqueous two-phase
total flavonoids
antioxidant activity
