摘要
考察了五种不同功能化磁性纳米粒子对Ru(bpy)^(2+)_3/TPA体系电化学发光(ECL)强度的影响,并对其中能显著增强ECL强度的磁性纳米粒子的使用条件进行了初步的优化。通过电化学发光检测技术发现BG-Fe、BPG-Fe、PAA-Fe、PE-Fe四种磁性粒子可增强Ru(bpy)_3^(2+)/TPA体系的ECL强度,增强幅度依次为30%、1 3%、73%、42%;COOH-MNPs磁性粒子可淬灭该体系的ECL强度,淬灭幅度为23.2%。实验结果表明,浓度为7μg/mL的PAA-Fe磁性纳米粒子经磁铁富集,在电极上富集5min是其促进ECL强度增强的最优条件。本研究对基于功能化磁性纳米粒子开发新的电化学发光试剂和建立新的电化学发光体系具有重要意义。
Herein, the effect of ifve different magnetic nanoparticles （MNPs） on electrochemiluminesence （ECL） intensity of the Ru（bpy）2＋3/TPA system were conducted. preliminarily operating conditions of the ideal MNPs were optimized.The result indicated that BG-Fe, BPG-Fe, PAA-Fe and PE-Fe could enhance ECL intensity in the Ru（bpy）2＋3/TPA system by method of ECL detection, and the enhanced amplitude of ECL intensity was 30%, 13%, 73% and 42%, respectively. COOH-MNPs could quench the ECL intensity and the amplitude was 23.2%. The utmost ECL enhancement of Ru（bpy）2＋3/TPA system was obtained in the optimum conditions as folows： PAA-Fe concentration of was at 7μg/ml and accumulation for 5 minutes by the magnet. The study is of important signiifcance for development of new ECL reagents and establishment of ECL system based on MNPs.
出处
《山东工业技术》
2014年第12期180-181,共2页
Journal of Shandong Industrial Technology
关键词
电化学发光
磁性纳米粒子
电化学发光试
Electrochemicaluminescence
Magnetic nanoparticles
ECL reagents
