摘要
目的：探讨纤溶酶原激活物抑制剂（PAI）-1活性及启动子区4G/5G基因多态性在静脉血栓栓塞症（VTE）发病机制中的作用。方法：收集我院2008-01-2011-06住院的VTE患者198例,其中维族86例,汉族112例;健康对照组212例,其中维族92例,汉族120例。采用PCR-PFLP技术检测PAI-1基因启动子区域的4G/5G多态性。结果：①PAI-1基因4G/5G的3种基因型在维族VTE组和对照组的分布频率分别为：4G/4G型（81.39%∶66.30%）、4G/5G型（15.12%∶21.74%）和5G/5G型（3.49%∶12.04%）;汉族VTE组和对照组基因分布频率分别为：4G/4G型（67.86%∶51.67%）、4G/5G型（19.64%∶29.17%）和5G/5G型（12.50%∶19.17%）,其基因型分布频率在同民族病例与对照组基因型分布比较,差异有统计学意义（P〈0.05）。②维汉两民族VTE组和对照组中,PAI-1基因4G/5G各基因型之间血浆PAI-1活性差异有统计学意义（P〈0.05）,其中4G/4G为基因型的血浆PAI-1活性高于同组同民族4G/5G、5G/5G型者。③通过非条件logistic回归模型校正后,PAI-1基因4G/4G基因型（OR=1.914,95%CI 3.345~5.708,P=0.001）及血浆PAI-1活性（OR=2.167,95%CI1.489~3.153,P=0.000）是维族VTE患者的独立危险因素,而吸烟（OR=2.867,95%CI 1.062~6.586,P=0.010）和血浆PAI-1活性（OR=1.357,95%CI1.141~1.614,P=0.001）是汉族VTE患者的的独立危险因素。结论：VTE患者血浆PAI-1活性均偏高,血浆PAI-1活性为维、汉两民族VTE患者的独立危险因素。维、汉两民族VTE组PAI-1基因4G/4G基因型频率较正常人群高,4G/4G基因型是维吾尔族VTE患者的独立危险因素。
Objective：To investigate the association between single nucleotide insertion/deletion （4G/5G） poly- morphism of PAI-1 gene and venous thromboembolism （VTE）. Method： The 198 cases including 86 Uygur and 112 Han ethnic diagnosed with VTE by the First Affiliated Hospital of Xinjiang Medical University between Janu- ary 2008 to June 2011 were admitted in this study. And 212 population including 92 Uygur ethnic and Han 120 ethnic were studied as controls. PCR-RFLP was applied to detect the polymorphism of PAI-1 gene 4G/5G poly- morphism in the promoter region. Result： The PAI-1 gene 4G/5G polymorphism distribution in Uygur VTE pa- tients and control groups were： 4G/4G （81.4% vs. 66.3%）, 4G/5G （15.1% vs. 21.7%） and 5G/5G（3.5% vs. 12%）, respectively; and in Han VTE patients and control groups were： 4G/4G（67.9%） vs. 51.7%）, 4G/5G （19.6% vs. 29.20//oo） and 5G/5G （12.5% vs. 19.2%）, respectively, and there were significant differences in dis- tribution of PAL1 4G/5G polymorphism between VTE patients and controls （P〈0.05）. Besides, plasma PAI-1 activity in PAI-1 gene 4G/4G genotype were statistically higher than 4G/SG and 5G/5G genotype （P〈0.05）. Multifactor logistic regression analysis showed that, PAI-1 gene 4G/4G genotype （OR= 1. 914, 95 % CI 3. 345- 5. 708, P=0. 001） and plasma PAI-1 activity （OR=2. 167, 95% CI 1. 489-3. 153, P=0. 000） were independent risk factors for Uygur patients while smoking （OR= 2. 867,95 % CI 1. 062-6. 586, P=0. 024） and plasma PAI-1 activity （OR=1. 357, 95% CI 1. 141-1. 614, P=0. 001） were independent risk factors for Han VTE patients. Conclusion：The plasma PAI-1 activity was higher in VTE patients compared to normal population in both Uygur and Han ethnic group, and plasma PAI-1 activity was an independent risk factor for VTE patients in Uygur and Han ethnics. The frequency of PAI-1 gene 4G/4G genotype was higher in VTE population than normal population, and 4G/4G genotype is an independent risk factor for Uygur VTE patients.
出处
《临床心血管病杂志》
CAS
CSCD
北大核心
2014年第7期576-582,共7页
Journal of Clinical Cardiology
基金
新疆重大疾病医学重点实验室-省部共建国家重点实验室培育基地项目(No:SKLIB-XJMDR-2012-7)
