摘要
建立东方草莓中（＋）-儿茶素的含量测定方法。采用AgilentZORBAXSB—C18色谱柱（4．6mm×150mm，5μm）分离；以乙腈-0．1％磷酸水为流动相进行梯度洗脱，流速为1mL／min，检测波长为280nm，对10批东方草莓中的（＋）-儿茶素进行了含量测定。结果表明：（＋）-儿茶素的保留时间为14．543min，其线性范围为0．0512～0．6400μg（R2=0．9999）；加样回收率为99．70％（RSD2．67％）。所建方法操作简单，稳定可靠，可用于东方草莓的质量评价。
The purpose was to establish the method of （ ＋ ）- catechin in Fragaria orientalis Lozinsk.The samples were performed on Agilent ZORBAX SB-C18 （4.6mm×150mm,5μm） column, eluted gradiently with mixture of acetonitrile-0.1 % phosphate solution at a flow rate of 1mL/min, and detected at 280nm.With this method, （ ＋ ）- catechin in iO batches of F. orienta/is Lozinsk.was determined, Results showed that （ ＋ ）- catechin retention time was 14.543min.The calibration curves had good linearity within the range of 0.0512-0.6400μg （R = 0.9999）.The average recovery of（ ＋ ）-catechin was 99.？0% （RSD 2.67% ）.The method was easy and stable, and could be used for the quantitative analysis of F.orientalis Lozinsk.
出处
《食品工业科技》
CAS
CSCD
北大核心
2014年第17期271-274,共4页
Science and Technology of Food Industry
基金
国家药典委员会项目(M11)
国家自然科学基金项目(21262047)
云南民族大学传统傣药药用物质基础研究创新团队
