摘要
通过丹参对外伤血瘀模型大鼠肾组织AQP2的调节效应,初步阐明丹参"活血利尿"的部分本质,为其临床调配用药提供指导。该文将30只SD大鼠随机分为正常组10只、血瘀证组20只,采用"定量化打击法"制备外伤血瘀证模型,再将造模动物随机分为模型组、丹参组各10只。治疗7 d后,免疫组化法、Western blot法检测大鼠肾组织AQP2蛋白表达、对比尿液AQP2含量,观察损伤局部肌肉、肾组织病理改变。结果,模型大鼠损伤局部肌肉炎性细胞浸润、血瘀并水肿,肾组织亦见瘀血肿胀且AQP2表达上调、尿AQP2含量增高,尿量减少,与正常组比较差异显著(P<0.05);而丹参组肾组织AQP2表达下调、尿AQP2含量减低,尿量增加,与模型组比较显著降低(P<0.05),肾组织肿胀明显减轻。表明丹参降低血瘀证大鼠尿液AQP2含量,下调肾组织AQP2蛋白表达,从而使肾小管对原尿中水的重吸收减少而增加尿量,丹参"活血"体现损伤局部组织消肿、也体现于肾脏的"利尿",减轻瘀肿。这一疗效与丹参调节肾脏AQP2效应密切相关。验证了丹参"活血利尿"效应的部分本质。
Partial nature of ″promoting blood circulation and dieresis″ of Salvia Miltiorrhizain was initially demonstrated by investigating the regulation effect of AQP2 expression in kidney of trauma blood stasis model rats with the Salvia Miltiorrhizain so as to provide guidance for its clinical deployment of administration. Random allocation was taken to averagely divide 30 SD rats into two groups： 10 rats in normal group and 20 rats in blood stasis syndrome group. Trauma blood stasis rat model was established by quantitatively beating. Then the rat model group was divided into model group and salvia group. After 7 days of treatment,the rat kidney AQP2 expression was detected,the content of urine AQP2 was compared and the damaged local muscle and kidney pathological changes were observed by immunohistochemical method and western blot method. Compared with that of the normal group,rats in model group had inflammatory cells infiltration,blood stasis and edema of the injured local muscles and up-regulated AQP2 expression,decreasing urinary output,and kidney tissues blood stasis and edema（ P 〈 0. 05）. On the other hand,compared with that of the model group,those parameters of rats in salvia group were all decreasing except urine output（ P 〈 0. 05）. Such result indicated that Salvia Miltiorrhiza can reduce trauma blood stasis rat content of urine AQP2 and down-regulated AQP2 expression in kidney tissue,so as to reduce the reabsorption of water by renal tubular and increase urine output. The promoting blood circulation effect of Salvia Miltiorrhizain can alleviate the degree of the damaged tissue edema and encourage urine drainage. This therapy is closely related to the effect of regulating AQP2 in kidney by salvia,so the purpose of this study by verifying ″promoting blood circulation and diuresis″ as the mechanism for the regulation effect of the salvia on AQP2 expression.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2014年第16期3162-3165,共4页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(81072742)
关键词
血瘀证
活血利水
活血利尿
丹参
AQP2
blood stasis syndrome
activating blood circulation and promoting water retention/promoting blood circulation and diuresis
Salvia Miltiorrhiza
AQP2
