摘要
利用重组AR（雄激素受体）基因酵母,结合土壤样品索氏提取、富集和净化方法,开展松花江流域吉林段土壤有机组分雄激素干扰效应及其分布特征研究.结果表明：所有样品均未检测出显著的AR诱导活性;部分样品检测出AR抑制活性,检出率为62.50%,最大抑制率为61.74%;采用RIC20（抑制率为20.00%时,96孔板每孔所需要的土壤当量,单位为mgwell）表征样品的抑制活性,RIC20在1.01-9.03 mgwell之间,表明松花江吉林段土壤中存在AR抑制活性物质,可能具有潜在的环境危害.研究区土壤雄激素干扰效应的分布特征表明,干扰效应分布与产业布局呈一定的相关性;工业区的AR抑制活性明显高于农田区,表明化工原料及产品的生产和使用及工业废水的排放可能是该地区土壤中AR抑制活性的重要来源.
Combined with Soxhlet extraction,enrichment and purification,a recombinant androgen receptor（ AR） gene yeast bioassay was conducted to assess the androgenic/anti-androgenic effects of the organic extracts from soil collected from the Songhua River basin in Jilin,China. The regional distribution characteristics of the androgenic/anti-androgenic effects were also analyzed. The results showed that the androgenic effects were not found in all soil samples. However,the anti-androgenic effects were found in 62. 50% of samples,and the maximum inhibition activity was 61. 74%. The value of RIC20（ the quantity of soil per well that could result in an inhibition activity equal to 20%） was calculated to evaluate the anti-androgenic effect,and was found to range from 1. 01 to 9. 03 mg/well. The results suggested that AR antagonists,which might have potential environmental risks,existed in the soil in the study area. The results also show that the distribution of the AR disrupting effects exhibited a positive correlation with the industrial layout in the study area. The anti-androgenic effects of the samples at the industrial areas were significantly higher than those at the agricultural areas,suggesting that the pollution from industries including the materials,products and wastewater may be important sources of anti-androgenic chemicals.
出处
《环境科学研究》
EI
CAS
CSSCI
CSCD
北大核心
2014年第8期898-903,共6页
Research of Environmental Sciences
基金
国家自然科学青年基金项目(41001351)
高等学校博士学科点专项科研基金资助课题(20100003120024)
关键词
雄激素受体
重组基因酵母
土壤
有机组分
雄激素干扰效应
androgenic receptor
recombinant gene yeast
soil
organic extracts
androgenic/anti-androgenic effect
