摘要
目的 观察胃癌组织中血管内皮生长因子（VEGF）、基质金属蛋白酶-2（MMP-2）的表达,探讨两者与胃癌侵袭、转移的关系.方法 采用免疫组织化学链霉菌抗生物素蛋白-过氧化物酶（SP）法检测30例胃癌癌前病变及42例胃癌组织中VEGF、MMP-2的表达.结果 VEGF在浅表性胃炎、慢性萎缩性胃炎伴肠化、不典型增生、早期胃癌和进展期胃癌中VEGF阳性表达率分别为25.0％ （3/12）、36.3％ （4/11）、42.8％（3/7）、57.1％ （8/14）、78.5％ （22/28）;MMP-2在浅表性胃炎、慢性萎缩性胃炎伴肠化、不典型增生、早期胃癌和进展期胃癌中MMP-2阳性表达率分别16.7％（2/12）、27.2％ （3/11）、28.6％（2/7）、42.8％ （6/14）、76.2％（20/28）.VEGF、MMP-2阳性表达率随着胃癌的发展逐渐升高.42例胃癌组织中,VEGF、MMP-2阳性率在侵及浆膜层组为73.1％和80.8％,显著高于未侵及浆膜层组（56.3％和62.5％,P＜ 0.05）;在淋巴结转移组为79.2％和87.5％,显著高于无淋巴结转移组（33.3％和44.4％,P＜0.05）;胃癌组织中VEGF、MMP-2表达呈正相关（r =0.416,P＜0.05）.结论 VEGF、MMP-2高表达与胃癌侵袭密切相关.
Objective To investigate the relationship between expression levels of vascular endothelial growth factor （VEGF） and matrix metalloproteinase （MMP）-2 and gastric cane invasion.Methods Streptavid-in-peroxidase （SP） immunohistochemical method was used to detect the expression of VEGF,MMP-2 in 30 cases of precancerous lesions and 42 cases of gastric carcinoma.Results The positive rate of VEGF in superficial gastritis,chronic atrophic gastritis with intestinal metaplasia,atypical hyperplasia,early gastric cancer,progressive stomach cancer were 25.0％ （3/12）,36.3％ （4/11）,42.8％ （3/7）,57.1％ （8/14）,78.5％ （22/28）.The positive rate of MMP-2 in superficial gastritis,chronic atrophic gastritis with intestinal metaplasia,atypical hyperplasia,early gastric cancer,progressive stomach cancer were 16.7％ （2/12）,27.2％ （3/11）,28.6％ （2/7）,42.8％ （6/14）,76.2％ （20/28）.With development of gastric carcinoma,the positive rate of VEGF,MMP-2 were increased gradually （P ＜ 0.05）.The positive rate of VEGF,MMP-2 in group of seroasinfiltration （73.1％,80.8％）were significantly high-er than that in group of outseroasinfiltration （56.3％,62.5 ％） （P ＜ 0.05）.The positive rate of VEGF,MMP-2 in group of lymph node metastasis （79.2％,87.5％）were higher than that in group without lymph node metastasis （33.3％,44.4％） （P ＜ 0.05）.There was a relationship between VEGF and MMP-2 expression （P ＜ 0.05）.Conclusion Both VEGF and MMP-2 contribute to the development,invasion and metastasis of gastric carcinoma.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2014年第8期1816-1818,共3页
Chinese Journal of Experimental Surgery
关键词
胃癌
血管内皮生长因子
基质金属蛋白酶-2
侵袭
Gastric cancer
Vascular endothelial growth factor
Matrix metalloproteinase-2
Invasion
