摘要
采用超声波辅助萃取法提取，高效液相色谱法测定了锁阳（Cynomorium songaricum）中的熊果酸含量。在单因素试验基础上，以乙醇体积分数、料液比、超声时间、超声功率为研究对象，进行了4因素3水平正交试验。结果表明，熊果酸在4.0~16.0μg/μL范围内呈良好线性关系，回归方程为y=622.7x-85.517，r=0.9998，加样回收率为98.08%。锁阳中熊果酸最佳提取工艺为：超声功率150 W，料液比1：25，85%（V/V ，下同）乙醇超声提取30 min，在此条件下，锁阳中熊果酸含量为1.53%。该工艺能有效提取锁阳中熊果酸，为评价中药材中的熊果酸水平提供参考。
Ursolic acid in Cynomorium songaricum was ultrasonically extracted and detected by HPLC. According to singlefactor experiments,three levels of four factors including ethanol concentration,material -liquid ratio,ultrasonic time, and ultrasonic power were selected for the orthogonal design.The results showed that the calibration curve of ursolic acid was linear （r=0.999 8） in the range of 4.0~16.0 μg/μL.The equation was y=622.7x-85.517,r=0.999 8,and recovery was 98.08%. The optimum extraction was carried out in 85%（V / V） ethanol for 30 min with the ultrasonic power of 150 W,and the material-liquid ratio 1：25.Under the optimal conditions,the content of ursolic acid was 1.53%.The method can extract ursolic acid efficiently,and provide a reference for evaluation ursolic acid in medicinal materials.
出处
《湖北农业科学》
北大核心
2014年第12期2894-2897,共4页
Hubei Agricultural Sciences
基金
北京市教育委员会科技发展计划面上项目(KM201000002004)
北京市属高等学校人才强教计划项目(PHR201107151)
