摘要
采用盆栽试验方法,考察自然干旱-复水处理下观赏植物红叶石楠、金叶女贞、小叶黄杨和大叶黄杨叶片超氧阴离子自由基(O-·2)产生速率、丙二醛(MDA)含量以及保护酶(SOD、POD、CAT)活性的变化,研究各种植物在干旱条件下的活性氧产生与酶促清除过程以及复水后的恢复情况差异,以揭示植物在逆境条件下的适应策略。结果表明:(1)自然干旱过程中,红叶石楠叶片O-·2生成速率呈先降低,之后明显升高,而小叶黄杨、金叶女贞、大叶黄杨均先升后降。(2)干旱提高了4种植物的MDA含量,但金叶女贞和大叶黄杨的MDA含量变化较小。(3)干旱下4种植物叶片的SOD活性增强,它们的POD活性变化不同,而CAT活性均先升高后下降。(4)复水后,4种植物O-·2产生速率和MDA含量均降低;小叶黄杨、金叶女贞、大叶黄杨SOD活性上升,而红叶石楠SOD活性下降;红叶石楠、金叶女贞、大叶黄杨CAT活性下降,而小叶黄杨则上升;红叶石楠、小叶黄杨、大叶黄杨POD活性下降,而金叶女贞却上升。研究表明,不同植物对活性氧的清除途径不同;隶属函数法分析表明4种观赏植物在干旱胁迫初期的综合抗旱性表现依次为:小叶黄杨>金叶女贞>大叶黄杨>红叶石楠。
Four ornamental species were planted in pots as the experimental material.They respectively are：Photinia fraseri,Buxus sinica （Rehd.et Wils.） Cheng var.parvifolia M.Cheng,Ligustrum × vicaryi Hort.and Euonymus japonicus Thunb.Natural drought-rehydration treatment was carried out in green houses.Physiological indicators of plants under drought stress and nature watering were determined,such as superoxide anion production rate,malondialdehyde （MDA）,activities of superoxide dismutase （SOD）,peroxidase（POD） as well as hydrogen peroxide enzyme（CAT）.Investigating the generation of active oxy gen and its enzymatic elimination process under drought stress and its recovery after rehydration will help us understand plant adaptation strategies to adversity and provide theoretical guidance for the scientific management of ornamental plants.The results showed that：（1）Under nature drought process,O2· genera ting rates of P.fraseri increased after shortly declined,while B.sinica var.parvifolia,Ligustrum × vicaryi and E.japonicus declined after shortly rising.（2）MDA content increased in P.fraseri and B.sinica var.parvifolia with little change in Ligustrum × vicaryi and Euonymus japonicus.（3）SOD activity of all the four species increases,while CAT activity declined after firstly increased.No trends were found about POD activity.（4）After rehydration,generation rates of O2 and MDA content of all the 4 species declined.SOD activity of B.sinica var.parvifolia,Ligustrum× vicaryi and E.japonicus increased while P.fraseri declined to control levels.CAT activity of Ligustrum× vicaryi increased,while all the other 3 species declined.As the POD activity,B,sinica increases with all the other 3 species declined.SOD and CAT activity promoted the elimination at first in P.fraseri.Different pathways for eliminating activated oxygen exist in the 4 ornamental plants.Subordinate function was used to rank their drought tolerance and the order was：B.sinica var.parvifolia＞Ligustrum × vicaryi＞E.japonicus＞P,fraseri.
出处
《西北植物学报》
CAS
CSCD
北大核心
2014年第7期1390-1396,共7页
Acta Botanica Boreali-Occidentalia Sinica
关键词
观赏植物
自然干旱复水
酶促清除机制
逆境生理
隶属函数
ornamental plants
natural draught-rehydration
eliminating mechanism under enzymatic promoting
stress physiology
subordinate function
