摘要
目的:观察水飞蓟素(silymarin)对实验性高脂血症模型小鼠脂质代谢的影响.方法:喂饲法建立高脂血症模型小鼠,实验设空白对照组、模型对照组、阳性对照组、水飞蓟素组和虎杖提取物组共5组,灌胃给药4 wk,每周称质量1次.4 wk后分别测定血清中总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)、高密度脂蛋白胆固醇(highdensity lipoprotein cholestero,HDL-C)、丙二醛(malondialdehyde,MDA)含量和谷丙转氨酶(alanine transaminase,ALT)、谷草转氨酶(aspartate transaminase,AST)、超氧化物歧化酶(superoxide dismutase,SOD)的活性.实时定量PCR和Western blot检测Sirt1基因的表达,以确定Sirt1的活性.结果:灌胃给药4 wk后,与空白对照组和模型对照组比较,阳性对照组、水飞蓟素组和虎杖提取物组均能够显著降低小鼠血液中TC、TG和MDA含量水平(P<0.05),升高血清中HDL-C水平和SOD活性(P<0.05);此外,阳性对照组、水飞蓟素组和虎杖提取物组中ALT、AST活性升高(P<0.05).PCR和Western b l o t结果显示阳性对照组和水飞蓟素组对Sirt1基因的表达具有抑制作用(P<0.05).而虎杖提取物对Sirt1基因的表达具有上调作用(P<0.05).结论:中药虎杖提取物及水飞蓟素成分有拮抗高脂血症的功效,但其作用的分子机制有一定的差异.
AIM： To investigate the effect of silymarin and Polygonum cuspidatum extract on lipid metabo- lism in mice with hyperlipidemia and the under- lying molecular mechanisms. METHODS： Mice were randomly divided into five groups： a control group, a model group, a positive group, a silymarin group and a Po- lygonum cuspidatum extract group. The control group was given a normal diet, while the other groups were fed a high-fat diet to induce hyper- lipidemia. After induction, the silymarin group and Polygonum cuspidatum extract group were intragastrically administered with silymarin and Polygonum cuspidatum extract for 4 wk, respec- tively, while the normal control and positive group were administrated with saline and simv- astatin, respectively. After 4 wk, all the rats were sacrificed and serum contents of total cholesterol （TC）, triglyceride （TG）, high-density lipoprotein cholesterol （HDL-C）, and malondialdehyde （MDA）, and activities of alanine transaminase （ALT）, aspartate transaminase （AST）, and super- oxide dismutase （SOD） were measured. Real- time quantitative PCR and Western blot were used to measure Sirtl expression. RESULTS： After treatment for 4 wk, TC, TG and MDA levels were significantly decreased, and HDL-C levels and SOD activity were sig- nificantly increased in the positive group, sily- marin group and Polygonum cuspidatum extract group compared with the model group （P 〈 0.05）. Compared with the model group, the activities of ALT and AST were significantly in- creased in the three treatment groups （P 〈 0.05）. PCR and Western blot results showed that sim- vastatin and silymarin had an inhibitory effect on Sirtl expression （P 〈 0.05）, while Polygonum cuspidatum extract promoted Sirtl expression （P 〈 0.05）. CONCLUSION： Silymarin and Polygonum cuspi- datum extract have therapeutic effects on hyper- lipidemia possibly via different mechanisms.
出处
《世界华人消化杂志》
CAS
北大核心
2014年第21期3072-3076,共5页
World Chinese Journal of Digestology
关键词
虎杖
水飞蓟素
高脂血症模型
脂质代谢
Polygonum cuspidatum
Silymarin
Hy-perlipidemia
Lipid metabolism
