摘要
目的探讨阻燃剂得克隆的环境激素作用。方法将去势Wistar雌鼠随机分为6组,去卵巢(模型)组、假手术组、得克隆低、中、高剂量组(25、50、100 mg/kg),阳性对照组(雌二醇50 mg/kg);连续灌胃14 d,第15 d处死,收集血清、摘取子宫称重并冷冻保存,通过电化学发光免疫法检测大鼠血清甲状腺激素(FT3、FT4、TSH)含量,采用Real-time RT PCR法检测子宫雌激素受体α(Erα)mRNA表达水平。结果与假手术组比较,模型组大鼠子宫重量[(0.112±0.035)g]与子宫/体重比值(0.072±0.030)明显降低,子宫内ErαmRNA表达水平明显下降(P<0.05);与模型组比较,高剂量得克隆组大鼠子宫重量[(0.419±0.059)g]与子宫/体重比值(0.186±0.143)明显上升,子宫内ErαmRNA表达量明显增加,差异有统计学意义(P<0.05);各组血清甲状腺激素水平无明显变化。结论得克隆在体内具有雌激素样作用,但对血清甲状腺激素无影响。
Objective To examine environmental hormone effects of flame retardant decholrane plus （DP）. Methods The ovariectomized（OVX） female Wistar rats were randomly divided into six groups：OVX group,DP treatment groups （25,50,100 mg/kg）, positive control group （ estradiol [E2], 50 mg/kg）, and sham-operated group. The rats were treated once a day for 14 consecutive days and sacrificed on day 15;then, the blood samples were collected and uterus were weighed and stored under frozen. Serum-free triiodothyronine （ FT3 ）, serum-free thyroxine （ FT4 ）, and thyroid stimulating hormone（TSH） levels were measured with electrochemiluminescence immunoassay and uterine estrogen receptor of （Erα） mRNA expression was determined with real-time reverse transcription PCR（RT-PCR）. Results Compared with the sham group,the uterine weight（0. 112±0. 035 g） and uterine/body weight ratio（0. 072 ± 0. 030） of the model group were significantly reduced and the expression of Erα mRNA level was significantly decreased （ P 〈 0. 05 ）. Compared with the model group,the uterine weight（0. 419 ±0. 059 g） and uterine/body weight ratio（0. 186 ±0. 143） of the high-dose DP group increased significantly and the expression of Erα mRNA in the uterus significantly increased, with significant differences （P 〈 0. 05 ）. No statisticallly significant changes in serum FT3 , PFT4, and TSH were observed in the rats with DP treatment. Conclusion DP has estrogenic effect but had no effect on serum thyroid hormone level in rats.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2014年第9期1163-1165,共3页
Chinese Journal of Public Health
基金
中央高校基本科学研究业务费专项资金(HIT.IBRSEM.2022003)
关键词
得克隆
环境激素
雌激素
dechlorane plus
environmental hormone
estrogen
